Chapter 6. Laboratory animal facilities Those who use animals for experimental and diagnostic purposes have a moral obligation to take every care to avoid causing them unnecessary pain or suffering. The animals must be provided with comfortable, hygienic housing and adequate wholesome food and water At the end of the experiment they must be dealt with in a humane manner
As aerosols are important sources of infection, care should be taken to reduce the extent of their formation and dispersion Hazardous aerosols can be generated by many laboratory operations, e.g. blending, mixing, grinding, shaking, stirring, sonicating and centrifuging of infectious materials. Even when safe equipment is used, it is best to carry out these operations in an approved biological safety cabinet whenever possible
A basic knowledge of disinfection and sterilization is crucial for biosafety in the laboratory. Since heavily soiled items cannot promptly be disinfected or sterilized, it is equally important to understand the fundamentals of cleaning prior to disinfection (precleaning). In this regard, the following general principles apply to all known classes of microbial pathogens
The Laboratory biosafety manual has in the past focused on traditional biosafety guidance for laboratories. The manual emphasizes the use of good microbiological work practices, appropriate containment equipment, proper facility design, operation and maintenance, and administrative considerations to minimize the risk of worker injury or illness. In following these recommendations, the risk to the environment and surrounding community-at-large is also minimized
Brucellosis is an infectious disease caused by the bacteria of the genus Brucella These bacteria are primarily passed among animals, and they cause disease in many different vertebrates Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals Humans become infected by coming in contact witl animals or animal products that are contaminated with these bacteria
