Restriction enzymes are used for cutting DNA at precise locations Vectors are used for the propagation of cloned DNA fragments Libraries of cloned DNA fragments allow searching for a specific DNA fragment
There are many ways of cloning a disease gene, depending on what is known about the disease. We will show several typical examples in this part of the lecture
Using mutants to analyze the pathway or functions of genes Mutants have to be generated to analyze the pathway or functions of genes Saturation screening Overexpression Suppressor mutations Synthetic lethals Nonallelic noncomplementation