Thin Layer Chromatography (TlC) is an extremely useful technique for monitorin reactions. It is also used to determine the proper solvent system for performing separations using column chromatography. tLC uses a stationary phase, usually alumina or silica, that is highly polar(standard) or non-polar(reverse phase). The mobile phase is a solvent whose polarity you will choose. In 5.301, and in most lab

Overview: As you may discover, growing single crystals takes patience as well as an artful hand Crystallizations can be very sensitive to temperature and minor disturbances. Therefore, you will be encouraged to try several different temperatures using otherwise identical conditions, and to always find a quiet undisturbed location to promote crystal growth. Here are some tried and true tips to get

Overview: A good 'H NMR sample contains about 10 mg of compound. The solution should contain no solids or paramagnetic impurities. Your deuterated NMR solvent should be free of water,and your NMR spectrum should contain no solvent peaks. Reference: Zubrick page 295 is relatively useful, but you should follow the specifics in this handout Also, keep in mind that we won't be running continuous wave spectrometers, so you should disregard any discussion of them. NMR Solvents:

This handout describes standard extraction and washing protocols that can be applied to virtually any crude reaction mixture. Aqueous washings are done to remove water soluble impurities from organic products since normally the compound that you desire will be dissolved in Reference

发现一些薄层分析法更复杂的技巧---剪玻璃TLC板,用火 焰造TLC染色剂点棍,使用TLC染色剂以使点显像。 Discover a few of the more complicatec skills related to thin layer chromatography cutting glass TLC plates, flame-pulling spotters, and using TLC stains to visualize spots

要熟悉流管柱层析法需要适当的练习。此影片将会让从你填充 的适当条件技巧开始、填充及进行管柱层析,甚至用管子做一个微 管柱层析。 It takes considerable practice to master the art of \running a column\. This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet

不要让不精确在实验室阻碍你!此影片介绍正确方法来使用滴 管、滴定管、量瓶和注射器精确测量液体体积。 Don't let inaccuracy hold you back in lab! This video introduces the proper methods for measuring precise volumes of liquid using pipets, burets, volumetric flasks, and syringes

使用旋转挥发仪:旋转挥发仪是你在实验室的好朋友。此影片 中将会确定你建立了一个安全并产生关系。 Using the Rotavap: The rotary evaporator is your friend in the lab. This video will ensure that you build a safe and productive relationship

从固态到气态,然后直接回到固态。此纯化技巧既漂亮又实用 的。找出此影片中为什么用大气压力来升华二茂铁(Ferrocene) From solid to gas, and then straight back to solid. This purification technique is both beautiful and useful. Find out why by watching the atmospheric pressure sublimation of ferrocene in this video

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