Lecture 7: Hydrogel Biomaterials: Structure and Physical Chemistry Last Day: programmed/regulated/multifactor controlled release for drug delivery and tissue engineering

engineering intracellular delivery Drug targeting Today biosensor device classes Detection methods Overview of biosensor technology Classes of biosensor devices External analysis/detection

Biodegradable Solid Polymers Spring 2003 Issued Day 1 Day 3 1. The chemical structure of a new degradable polymer, poly (ethylene glycol)-b-polylysine-b- poly (D, L-lactide)

Biodegradable Solid Polymers Spring 2003 Issued Day 1 5 points/problem(20 points total) 1. The chemical structure of a new degradable polymer, poly(ethylene glycol)-b-polylysine-b- poly (D, L-lactide), is shown below a. Based on chemical structure al

1. An early study in the development of peptide-presenting biomaterials showed that polymer surfaces bearing RGD peptides at a density equivalent to -10 peptides per each cell was sufficient to promote cell attachment, spreading and subsequent cell growth. In contrast, when whole

1. Re-derive the equation for equilibrium swelling of a polyelectrolyte gel where the polymer chains contain a basic ionizable group that becomes positively charged with decreasing pH

1. Re-derive the equation for equilibrium swelling of a polyelectrolyte gel where the polymer chains contain a basic ionizable group that becomes positively charged with decreasing pH

detection methods Surface plasmon resonance biosensors Today cell- and tissue-based sensors Primary transducers and biosensor design with living cells microphysiometer Reading J.J. Pancrazio et al., Development and application of cell-based biosensors, Ann Biomed

Lecture 17: Drug targeting Last time Intracellular drug de Today Drug targeting Reading T.J. Wickham, 'Ligand-directed targeting of genes to the site of disease, Nat. Med. 9(1) 135-139(2003)

Example: HIV-1 DNA vaccine delivered with boosters to elevate Ab titers2: Mechanical and electrical devices that can provide digitized release typically require larger devices and surgical implantation(e.g. Pharm. Res. 1, 237(1984); also have high cost