Cloning vectors: 克隆载体 to clone a gene in a vector Expression vectors: 表达载体 to express a gene from a vector Integration vectors: 整合载体 to integrate a gene in a genome through a vector Cloning vectors 1 Plasmid vecters 2 Bacteriophage vectors 3 Cosmids & BACs 4 Eukaryotic vectors Cloning vectors: allowing the exogenous DNA to be inserted, stored, and manipulated mainly at DNA level. expression vectors: allowing the exogenous DNA to be inserted, stored, and expressed
1 Motivation The Poisson problem has a strong formulation a minimization formulation and a weak formulation T weak formulations are more general than the strong formulation in terms of regularity and admissible data SLIDE 2 The minimization/weak formulations are defined by: a space X; a bilinear The minimization/weak formulations identify ESSENTIAL boundary conditions NATURAL boundary conditions ed in a The points of departure for the finite element method are the weak formulation(more generally) the minimization statement (if a is SPD) 2 The dirichlet problem 2.1 Strong Formulation Find u such that
