Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer
Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers
Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures