上海交通大学：通识教育核心课程《生命科学发展史 A History of Life Science》教学资源（课件讲稿）绪论（孟和）

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

1.DNA Structure 2.DNA Topology 3.RNA Structure

Part I: Chemistry and Genetics Part II: Maintenance of the Genome Part III: Expression of the Genome Part IV: Regulation Part V: Methods

1. RNA polymerase and transcription cycle 2. The transcription cycle in bacteria 3. Transcription in eukaryotes •Molecular Biology Course

Quiz: Section H, I and J, 30 min, English answer only 1. What are the essential elements that a cloning vector should have? how about the expression vectors and the integration vectors? What is a shuttle vector and please describe its importance? (35)

1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

（1）开卷考试，个人独立完成 （2）不须偷看别人的答案 （3）老师宣布收试卷时，试卷必须快速由每排的最左

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary