Overview: A good 'H NMR sample contains about 10 mg of compound. The solution should contain no solids or paramagnetic impurities. Your deuterated NMR solvent should be free of water,and your NMR spectrum should contain no solvent peaks. Reference: Zubrick page 295 is relatively useful, but you should follow the specifics in this handout Also, keep in mind that we won't be running continuous wave spectrometers, so you should disregard any discussion of them. NMR Solvents:

Flash column chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in 5.301,around 1.0 g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column chromatography uses the same principles

This handout describes standard extraction and washing protocols that can be applied to virtually any crude reaction mixture. Aqueous washings are done to remove water soluble impurities from organic products since normally the compound that you desire will be dissolved in Reference

说明: 仪器分析是我校化学专业开设的一门应用性的专业基础课程。开设的目的是使学 生在学习分析化学的基础上,学习和掌握仪器分析的基本原理、基本方法、特点和应 用范围,了解现代分析仪器的发展方向。加强对其它课程所学有关知识和实验操作技 能的理解和应用。开阔学生的知识面。提高学生适应各种工作的能力,为今后的工作 打好基础

大肠杆菌常规培养 大肠杆菌在LB( Luria-Bertani-)液体培养基中,37℃水浴摇床培养12-16小时在LB固 体培养基中,置于37℃培养箱培养12-16小时。如果培养用于提取具有氨苄青霉素(Amp)抗性 基因质粒(如pUC18)的菌体,则需在培养基中加入100mg/ml的氨青霉素

遗传学是研究生物遗传和变异规律、探索生命奥秘的理论科学,是高等农业院校所设的 一门重要的专业基础课,是植物、动物和微生物育种及相关课程的理论基础。 《普通遗传学》是农学、园艺、园林专业的必修专业基础课。通过本课程学习,要求学 生掌握遗传学的基本原理,掌握对动、植物和微生物进行遗传分析的一般方法,掌握基本的 实验操作技术,为进一步学习有关专业课程和遗传学的分支学科奠定较好的遗传学基础知识

WARNING NOTICE: The erperimants described in these matcrials are potantially hazardous and require a high level ofsafcty training special facilities and equipment, and supervision by appro responsibility, liability, and risk for the implemontation of such safey procedures and meastncs. MIT shall have no responsibility, liability, or risk for the content or implementation any of the matainl presented. Legal Notices

Chemists use spectroscopy (Ir, UV-Vis, NMR) to determine molecular structure. spectroscopy: technique that measures the amount of radiation a substance absorbs at various wavelengths(based on the quantization of molecular energy levels Molecules exist in quantized energy states (rotational, vibrational, and electronic quantum states) Molecules absorb discrete amounts of energy(ae)and are excited to higher

equipment and supavision by appropriate individuak You bear the sol responsibility, liability, and risk for the implemcntation of such safety procedures a ares. MIT shall have no responsibility, liability, risk for the content or implementation of of the mat

发现一些薄层分析法更复杂的技巧---剪玻璃TLC板,用火 焰造TLC染色剂点棍,使用TLC染色剂以使点显像。 Discover a few of the more complicatec skills related to thin layer chromatography cutting glass TLC plates, flame-pulling spotters, and using TLC stains to visualize spots