Overview: A good 'H NMR sample contains about 10 mg of compound. The solution should contain no solids or paramagnetic impurities. Your deuterated NMR solvent should be free of water,and your NMR spectrum should contain no solvent peaks. Reference: Zubrick page 295 is relatively useful, but you should follow the specifics in this handout Also, keep in mind that we won't be running continuous wave spectrometers, so you should disregard any discussion of them. NMR Solvents:

Flash column chromatography is a quick and (usually) easy way to separate complex mixtures of compounds. We will be performing relatively large scale separations in 5.301,around 1.0 g of compound. Columns are often smaller in scale than this and some of you will experience these once you move into the research lab. Column chromatography uses the same principles

萃取、冲洗和干燥:还没到结束不能算结束。藉由分液漏斗示 。 范液相-液相萃取并学习如何用”反应检查步骤”检查你的反应。这 是一个你不会想错失的一个纯化技巧! Extracting, Washing and Drying: It ain't over 'til it's over. Learn how to \work up\ your reaction using a separatory funnel to perform a liquid-liquid extraction. This is one purification technique you don't want to miss!

Chemists use spectroscopy (Ir, UV-Vis, NMR) to determine molecular structure. spectroscopy: technique that measures the amount of radiation a substance absorbs at various wavelengths(based on the quantization of molecular energy levels Molecules exist in quantized energy states (rotational, vibrational, and electronic quantum states) Molecules absorb discrete amounts of energy(ae)and are excited to higher

WARNING NOTICE: The erperimants described in these matcrials are potantially hazardous and require a high level ofsafcty training special facilities and equipment, and supervision by appro responsibility, liability, and risk for the implemontation of such safey procedures and meastncs. MIT shall have no responsibility, liability, or risk for the content or implementation any of the matainl presented. Legal Notices

发现一些薄层分析法更复杂的技巧---剪玻璃TLC板,用火 焰造TLC染色剂点棍,使用TLC染色剂以使点显像。 Discover a few of the more complicatec skills related to thin layer chromatography cutting glass TLC plates, flame-pulling spotters, and using TLC stains to visualize spots

described in these materials are potentially hazardous and require a high lcvel ofsafity training special facilitics and equipment, and supavision by approprinte duals. You bear the sol responsiblity, liability, and risk for the impkemcntation of such safety procedures and measures. MiT shall have no responsibility, liability, ar risk for the content ar implem of ay of the matcrial presented. Legal Notices

将液体从固体中分开来的最简易方法是什么?过滤在此影片中 将学习如何有效的用重力过露及真空过滤来进行。 The easiest way to separate a liquid from a solid? Filtration! Learn how to effectively carry out gravity and vacuum filtrations in this video

This is two-day- experiment. Do not start this experiment unless you have two free lab days to complete it.*** Techniques Checklist: Picking the correct eluent Adsorbtion of a crude mixture onto silica gel Separating complex mixtures using gradient elution Pre-lab Discussion: Suggest limited list of eluent solvent systems Discuss sample adsorption and gradient elution strategies

described in these matorials arc potentinlly hazandous and require a high levd ofsafety training spccial facilitics and equip mant, and supervision by approp iate individuals. You bea the sole responsibility, lisbility, and risk for the implamnentation of such safcty procedures and mcasures. MIT shall have no respongibility, liability, or risk for the content or implementationc any of the material presanted. Legal Notices