Chapter 5 Macromolecules and Molecular Genetics DNA Replication Complementary base pairing Semiconservative Template and primer From 5'-phosphate to the 3'-hydroxyl end DNA polymerase and ligase A short stretch of RNA serves as primer Primase: a specific RNA-polymerizing enzyme
Peptidoglycan Synthesis Transport of peptidoglycan precursors across the cytoplasmic. membrane to the growing point of the cell wall Outside Peptidoglycan Growing point of cell wall
Chapter 4 Metabolism, Biosynthesis and Nutrition Microbial Nutrition Laboratory Culture of Microorganisms Sterilization. Aseptic Technique, and Pure Cultures
Genetic Engineering and Biotechnology Genetic engineering: Isolation manipulation and expression of genetic materials. Gene cloning: Isolation and purification of specific genes In vitro recombination=recombinant DNA
Chapter 7 Regulation of Gene Expression Regulation of Enzyme Activity Constitutive enzymes -Enzymes induced by specific circumstances Two major modes of regulation in the cell Control the activity of preexisting enzymes Control the amount of an enzyme
Questions for Eukarya 1. List the Eukaryotic Microorganism you have learned. 2. Describe the differences between various eukaryotic microorganisms. 3. What do algea have various colors? 4. How to classify algae? 5. List six major algae and describe their characteristics you have learned?