A remarkab|efie|d e One of the two leading technologies in structural biolog y Great contributions to biology A special window to the development of modern structural biology and biophysics THNMR YAN

3 Multidimensional NMR Spectroscopy C Gerd Gemmecker 1999 Models used for the description of NMR experiments 1. energy level diagram: only for polarisations, not dependent phenom

4 DQF-COSY, Relayed-COSY, TOCSY Gerd Gemmecker 1999 Double-quantum filtered cOSY The phase problem of normal COsY can be circumvented by the dQF-COSY, using the MQc term generated by the second 90 pulse Ilz cos( @2t1) cos( t1) polarization

NMR supplement Hendrickson, W-A& Wuthrich, K Macromolecular 16. Leahy, D.J., Aukhil, I.& Erickson, H.P. 2.0 Cell 84 A K.D. thanks the Wellcome Trust and The queens 2D0i9 Riddiough. G. Nature Struct. Biol 18. sakaning. AK Kean. cel l8r.597-605i(eRw& by the Wellcome Trust. The authors acknowledge

3. 1 Chemical shift When an atom is placed in a magnetic field B its electrons circulate about the direction of the applied magnetic field. This circulation causes a small magnetic field at the nucleus which opposes the externally applied field The magnetic field at the nucleus(the effective field) is therefore generally less than the applied field by a fraction THNMR

NMR supplement Kinetic studies of protein folding using NMR spectroscopy Christopher M. Dobson and Peter J Hore Recent progress has advanced our abilities to use NMR spectroscopy to follow- in real time -the structural and dynamic changes taking place during protein folding

6 The noe effect c Gerd Gemmecker, 1999 Nuclear OVERHAUSER Effect(NOE): resonance line intensity changes caused by dipolare cross- relaxation from neighbouring spins with perturbed energy level populations To understand the nature of the NOE, we have to look at a two-spin system I' and

NMR spectroscopy in structure-based drug design Gordon CK Roberts NMR methods for the study of motion in proteins continue Structure and dynamics of the binding site improve, and a n d complexes Methodological developments in NMR of macromolecules relevant to drug design

nature publishing group http://structbio.nature.com news and views Department of Biological Sciences. chem.276146-116200 Columbia University, New York, New York 6. Chen,.. Sharma, 5. Quiocho, F.A. Davidson. A.L. 15. Jones. P.M. George. A.M. FEMS Microbiol. Lett

• 量子力学基础 • 密度算符 • 脉冲及其算符描述 • 核磁信号计算 • 多自旋体系的描述 • 相干 • 乘积算符