1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

（1）开卷考试，个人独立完成 （2）不须偷看别人的答案 （3）老师宣布收试卷时，试卷必须快速由每排的最左

Q1: Please name the following amino acids (three letter name, for example: Ala for alanine). Q2: Which of them are hydrophobic AA, and which are hydrophilic?

Part I: Describe the following 10 terms (3 points each). (1) EF-G: a. its structure likes tRNA b. it is a translational elongation factor c. it binds to ribosome when associated with GTP

1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)

Describing the following terms (4 points each) 1. Cre recombinase 2. CSSR recombination 3. Mating-type switching 4. Mismatch repair 5. Polymerase switching 6. 30-nm fiber 7. Core histones

Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. 1. Yeast artificial chromosome 2. RNA interference 3. Proteomics

PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary

Recombinant gene expression in eukaryotic systems is often the only viable route to the large-scale production of authentic, post￾translationally modified proteins. It is becoming increasingly easy to find a suitable system to overexpress virtually any gene product

How Important Is Cost? Reagents obviously figure into the cost of a procedure, but the labor required to produce and apply the reagents of purification should also be considered. How Important Is Reproducibility (Robustness) of the Procedure?