1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)

1、营销渠道是什么(What is the nature of marketingChannels)？ 2、公司在设计、管理、评价和修正其渠道时将面临什么决策(What decisions do companies face in designing,managing, evaluating, and modifying their channels)？ 3、渠道的动态发展趋势是什么(What trends are takingplace in channel dynamics)？ 4、如何管理渠道的冲突(How can channel conflict bemanaged)？

Utilitarianism Last time we considered three questions one might ask an ethical theory to answer i)Which acts are right and which are wrong? Which acts ought we to perform(understanding the\ought as a moral ii)What makes a particular action right or wrong? What is it about the action that determines its moral status? 111) How do we know what is right and wrong? There are a variety of strategies for answering(iii). One might hold e. g. that moral truths are revealed by god and

Defining the Field-what do you think that economics is? (1) Subject Matter Based Definitions (2)UItra-pragmatic definitions( what economists do) (3)Methodology Based Definitions This much is certain a. Economics pretty much includes all topics related to human society

Questions on the text 1. Why and how do people change their attitude towards environment? 2. How did Canada protect its fish supply and what was the result? 3. What measures did Costa Rica take to protect its remaining forest? 4. What does the writer think of Brazil's environment protection?

选择和管理营销渠道 1、营销渠道是什么(What is the nature of marketing Channels)? 2、公司在设计、管理、评价和修正其渠道时将面临什么 (What decisions do companies face in designing, managing, evaluating, and modifying their channels)? 3、渠道的动态发展趋势是什么(What trends are taking place in channel dynamics)? 4、如何管理渠道的冲突(How can channel conflict be managed)?

al Identity ll Problem of synchronic identity for persons: under what conditions are two simultaneous person-events events in the life of the same person? Problem of diachronic identity for persons under what conditions are two person-stages stages in the life of a single person. In particular, what makes a particular person-stage a continuation of me as I am right now? Background desiderata: an account of personal identity should allow for the possibility of anticipation and memory, i.e., of individual psychological connections between different person stages. It should also allow us to justify our practices of

Ⅱ. Listening skills W: From what i can remember the director asked us to rehearse this a hundred times M: One hundred times? Is the director out of his mind Q: What does the man imply? 2. M: Do you think we have made enough food for the party? w: The refrigerator is about to explode Q: What does the woman imply? 3. w: She said she might become a famous movie star M: Yes, and pigs might fly

Leading-in Activities: Discussion 1. What's your definition of money? 2. What attitude should we take toward money? 3.What’ your fantasy?

Ⅱ. Listening skills M: The police gave a few tickets out last week along Highway 15 w: In fact, quite a few tickets were given on that road Q: What does the woman mean? 2. M: Who do you think is the smartest student in the class? w: Mary is second to none Q: What does the woman say about Mary 3. W: What are you so happy about?