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1. INTRODUCTION 2. SAMPLE IDENTIFICATION & DATA MANAGEMENT FOR MICROBIOLOGY SAMPLES 3. RATE AND ACTIVITY MEASUREMENTS 4. COUNTING METHODS 5. BIOMASS AND BIOMARKER METHODS 6. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: CLONE LIBRARIES 6.1. DNA EXTRACTION FROM SEDIMENTS 6.2. PCR 6.3. SELECTING AND CONFIRMING EFFICACY OF 16S RRNA GENE PRIMERS 6.4. SELECTING AND USING PRIMERS FOR FUNCTIONAL GENES 6.5. CLONING, CLONE SELECTION AND SEQUENCING 6.6. SCREENING CLONE LIBRARIES FOR ARTEFACTS, CHIMERAS AND COVERAGE 6.7. PHYLOGENETIC ANALYSES 7. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: PROFILING METHODS 7.1. DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE) FOR 16S RRNA GENES 7.2. BENTHIC DIVERSITY PROFILING OF BACTERIA USING ARISA 7.3. TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) ANALYSIS 8. CULTURE METHODS 9. REFERENCES: FULL LIST
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《DNA生物合成》PPT教学课件:A nucleic acid consists of four kinds of bases linked to a sugar-phosphate backbone
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◼ 前言 ◼ 诱发基因突变的因素 ◼ 基因突变的一般特性 ◼ 基因突变的分子机制 ◼ DNA损伤的修复
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《植物博物学:用洗手液提取植物的DNA》课件
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1.The Exploration of Genes Relies on Key Tools 2.Recombinant DNA Technology Has Revolutionized All Aspects of Biology 3.Complete Genomes Have Been Sequenced and Analyzed 4.Eukaryotic Genes Can Be Manipulated with Considerable Precision
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1. Biochemical Unity Underlies Biological Diversity 2. DNA Illustrates the Interplay between Form and Function 3. Concepts from Chemistry Explain the Properties of Biological Molecules 4.The Genomic Revolution Is Transforming Biochemistry and Medicine
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上海交通大学:《生物化学 Biochemistry(B类)》课程教学资源(题库与答案)Chapter 4 DNA, RNA, and the Flow of Genetic Information
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10.6 Genetic recombination遗传重组 10.7 Transformation 10.8 Transduction 转导 10.9 Conjugation: Essential Features 10.10 The Formation of Hfr Strains and Chromosome Mobilization 10.11 Mobile DNA: Transposable Elements转座因子
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I. Overview of Regulation 8.1 Major modes of regulation II. Regulation of Enzyme Activity (Posttranslational level regulation) 8.2 Noncovalent enzyme inhibition 酶活的非共价抑制 8.3 Covalent Modification of Enzyme 酶的共价修饰 III. DNA-Binding Proteins and Regulation of Transcription by Negative and Positive Control (Transcriptional level regulation) 转录水平调控
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上海交通大学:《微生物学 Microbiology》课程教学资源(双语课件)Lecture 11.1 Genetic Engineering(Methods for Manipulating DNA)
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