1. INTRODUCTION 2. SAMPLE IDENTIFICATION & DATA MANAGEMENT FOR MICROBIOLOGY SAMPLES 3. RATE AND ACTIVITY MEASUREMENTS 4. COUNTING METHODS 5. BIOMASS AND BIOMARKER METHODS 6. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: CLONE LIBRARIES 6.1. DNA EXTRACTION FROM SEDIMENTS 6.2. PCR 6.3. SELECTING AND CONFIRMING EFFICACY OF 16S RRNA GENE PRIMERS 6.4. SELECTING AND USING PRIMERS FOR FUNCTIONAL GENES 6.5. CLONING, CLONE SELECTION AND SEQUENCING 6.6. SCREENING CLONE LIBRARIES FOR ARTEFACTS, CHIMERAS AND COVERAGE 6.7. PHYLOGENETIC ANALYSES 7. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: PROFILING METHODS 7.1. DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE) FOR 16S RRNA GENES 7.2. BENTHIC DIVERSITY PROFILING OF BACTERIA USING ARISA 7.3. TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) ANALYSIS 8. CULTURE METHODS 9. REFERENCES: FULL LIST

一、填空题(将你认为最恰当的词句填在空格上,使句意完整通顺) 1生理学的研究水平大致可分为 水平、 水平和 水平等。 2.易化扩散有两种类型:

13.1数量性状的概念与特点 13.2数量性状的遗传基础 13.3数量性状分析的基本方法 13.4遗传参数的估测及其应用

14.1概念 14.2纯系学说 14.3近交与杂交的遗传效应 14.4杂种优势 14.5近交与杂交在育种上的应用

11.1细胞质遗传的概念和特点 11.2母性影响 11.3线粒体和叶绿体遗传 11.4植物雄性不育性的遗传 11.5其他细胞质遗传因子

6.1染色体结构变异的概述 6.2染色体结构变异类型 6.3染色体结构变异的诱发 6.4染色体结构变异的应用

基本名词概念 3.1分离定律 3.2自由组合定律 3.3数理统计原理在遗传研究中的应用 3.4 Mendel定律的扩展 3.5孟德尔遗传规律的意义 3.6人类系谱图式

Restriction enzymes are extensively used in molecular biology. Below are the recognition sites of two of these enzymes, BamHI and Bcll. a)BamHI, cleaves after the first G: 5'GGATCC 3 3 CCTAGG5 Does cleavage by BamHI result in a 5' or 3 overhang? What is the sequence of this overhang?

Please print out this problem set and record your answers on the printed copy. Answers to this problem set are to be turned in at the box outside by 4: 10 Wednesday, October 8. Problem sets will not be accepted late Solutions will be posted on the web

Please print out this problem set and record your answers on the printed copy. answers to this problem set are to be turned in at the box outside by 4: 10 Wednesday, November 26. Problem sets will not be accepted late Solutions will be posted on the web November 27