西北工业大学理学院：《工科物理教程》 3-6-7 演示实验图片——有极分子介质极化模型

第四章热学基础 1.了解分子动理论基本观点和理想气体模型 2.从微观分子运动角度领会宏观量压强、温度、内能、熵的含义

(1)甲烷 (2)乙烷 (3)环己烷 (4)异庚烷

Molecular Biology of the Gene,5/e--- Watson et al.(2004) Part: Chemistry and Genetics Part ll: Maintenance of the Genome Part Ill: Expression of the Genome Part IV: Regulation Part V: Methods

Collaboration of Proteins at the Replication Fork Leading strand DNA polymerase lll Leadi ng strand template Lagging strand RNA primase template RNA primer

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??