THE3rd EXPERIMENTIn situ display of DNA andRNA in the cell
THE 3rd EXPERIMENT In situ display of DNA and RNA in the cell
[Aims]To master the method of in situ display ofDNA and RNA in the cellTo learn the method of the blood smear,To know the process of fixation and dyeing
[Aims] ⚫ To master the method of in situ display of DNA and RNA in the cell ⚫ To learn the method of the blood smear, ⚫ To know the process of fixation and dyeing
[principles]DNA and RNA could be dyed differentcolors by the solution mixed withmethylgreen and pyronine because of thedifferent polymeric degree. DNA is dyedto be green and blue by methylgreen forits high polymeric degree, whereas RNAis dyed to be red by pyronine for its lowpolymeric degree
[ principles ] ⚫ DNA and RNA could be dyed different colors by the solution mixed with methylgreen and pyronine because of the different polymeric degree. DNA is dyed to be green and blue by methylgreen for its high polymeric degree, whereas RNA is dyed to be red by pyronine for its low polymeric degree
[Contents and ManipulationsPreparing a smear of blood cells fromthe toadPut the toad into death -→open the coelom andpericardiac muscle by the scissors → dip one drop ofblood near the end of a clean slide →press the blooddrop with the end of the other slide-→ push the twoslides in opposite direction → make the blood dropform blood membrane→air dry it
[Contents and Manipulations] 1. Preparing a smear of blood cells from the toad ⚫ Put the toad into death open the coelom and pericardiac muscle by the scissors dip one drop of blood near the end of a clean slide press the blood drop with the end of the other slide push the two slides in opposite direction make the blood drop form blood membrane air dry it
FixingPlace the blood smear into 70% ethanol for 5-10mins, then air dry it .Dyeing3.Put the slide into the solution mixed withmethylgreen and pyronine for 10-15mins,then wash it by tap water.ExaminingObserve the slide under light microscope
2. Fixing ⚫ Place the blood smear into 70% ethanol for 5- 10mins, then air dry it . 3. Dyeing ⚫ Put the slide into the solution mixed with methylgreen and pyronine for 10-15mins, then wash it by tap water. 4. Examining Observe the slide under light microscope
Thereportofthe experimentThe titleThe contentDraw two cells to show DNA and RNAHand it in in class
⚫ The title ⚫ The content ⚫ Draw two cells to show DNA and RNA ⚫ Hand it in in class. [The report of the experiment]
.3-4 students are arranged by themonitor to clean the classroom●Collect the garbage to the ash binwhich is at the corner nearby thetoilet of this floor
⚫3-4 students are arranged by the monitor to clean the classroom ⚫Collect the garbage to the ash bin which is at the corner nearby the toilet of this floor