复旦大学：《基因组学》课程教学资源（学习资料）人类基因组范围转录异构变异——表达水平多样性

LETTERS natire enetics Genome-wide analysis of transcript isoform variation in humans Tony Kwan 2, David Benovoyl, 2, Christel Dias, Scott Gurd, Cathy Provencher, Patrick Beaulieu,, s Thomas J Hudson,2, 4, Rob Sladek 2& Jacek Majewski 2 We have performed a genome-wide analysis of common functional relationships to diseases. However, little is known at present genetic variation controlling differential expression of about the genetic variation at the sub-transcript level or about transcript isoforms in the CEU HapMap population using a differences in multiple transcript isoforms of the same gene. Here, splicing differences between various types of samples.sing the 9 comprehensive exon tiling microarray covering 17, 897 genes. we interrogated transcripts across their entire length, usi We detected 324 genes with significant associations between Affymetrix GeneChip Human Exon 1.0 e flanking SNPs and transcript levels. Of these 39% reflected s changes in whole gene expression and 55% reflected transcript Exons within a gene are represented on the microarray by indivi- dual probe sets, and were considered discrete units for our analysis of initiation of transcription)use, and diene, differential 5 UTR transcript isoform-processing differences. We used triplicate samples of lymphoblastoid cell lines(LCLs)derived from 57 unrelated Centre d'Etudes du Polymorphisme Humain(CEPH) CEU individuals(Utah a that the regulatory effects of genetic variation in a normal e human population are far more complex than previously residents with northern and western European ancestry) genotyped by the HapMap consortium, allowing us to establish a possible genetic observed. This extra layer of molecular diversity may account basis for any observed variations in transcript isoforms with associated for natural phenotypic variation and disease susceptibility SNPs. A linear regression analysis under a codominant model was carried out to associate probe set expression intensities with the N Alternative pre-mRNA processing increases the complexity of eukary- genotypes of all SNP markers within a window of 50 kb flanking o otic transcriptomes, allowing multiple transcripts and protein iso- the boundaries of the transcript cluster(meta-probe set)containing forms with distinct functions to be produced from a single genomic the probe set. We assessed the statistical significance of th e vanation locus. Within an organism, tissue specific gene isoforms are known to using the t-statistic, and used the regression equation to estimate the have important functions in development and proper functioning of fold change in expression between the two homozygous genotypes. We diverse cell types. Across individuals, changes in normal isoform used permutation testing 8 to determine empirical P-values corre- structure have phenotypic consequences and have been associated sponding to the asymptotic P-values obtained from the regression with disease. Splicing defects in a number of genes, such as the cystic Subsequently, we applied the false discovery rate(FDr)correction to fibrosis transmembrane conductance regulator, CFTR, result in several establish a cutoff P-value of 9. x 10, corresponding to the 0.0 known mendelian disorders. More subtle changes, such as alternative FDR level(see Methods). This yielded 757 unique probe sets showing 3 processing and polyadenylation, have recently been associated with significant SNP associations, belonging to 317 unique meta-probe sets complex disorders: OASI in severe acute respiratory syndrome, (Supplementary Table I online). Although the most significant SNPs TAP2 in type I diabetes, and IRF5 in susceptibility to systemic may not e causative polymorphisms responsible for these differences in probe set expression, they are very probably in linkage Several recent studies have suggested that natural variation at the disequilibrium with the causative polymorphism(s). This is reflected level of whole-gene expression is common in humans and is associated in the distance distribution of associated polymorphisms, most of with genetic variants, such as SNPs or copy number variants which are in close proximity to the probe sets( Supplementary Fig. 1 (CNVs)0-13. Studying variation in gene expression is becoming online). The association analysis at the transcript(meta-probe set) increasingly important because of its contribution to phenotypic level resulted in a 0.05 FDR cutoff of 6.02 x 10, yielding 127 unique differences among individuals and its possible regulatory and transcripts with significant genetic association at the gene expression dEpartment of Human Genetics, McGill University and University and Genome Quebec Innovation Centre, 740 Dr. Penfield, Room 7210, Montreal, Quebec H3A 1A4, Canada. Division of Hematology-oncology, te-Justine Hospital, Montreal, Quebec H3T lC5, Canada. Ontario Institute for Cancer College Street, Suite 800, Toronto, Ontario M5G lL7, Canada. Correspondence should be addressed to M. jacek majewski@mcgill. ca). Received 9 July 2007; accepted 31 October 2007: published online 13 January 2008; doi: 10. 1038/ng. 2007.57 NATURE GENETICS VOLUME 40 NUMBER 2 I FEBRUARY 2008

Genome-wide analysis of transcript isoform variation in humans Tony Kwan1,2, David Benovoy1,2, Christel Dias1, Scott Gurd2, Cathy Provencher2, Patrick Beaulieu3, Thomas J Hudson1,2,4, Rob Sladek1,2 & Jacek Majewski1,2 We have performed a genome-wide analysis of common genetic variation controlling differential expression of transcript isoforms in the CEU HapMap population using a comprehensive exon tiling microarray covering 17,897 genes. We detected 324 genes with significant associations between flanking SNPs and transcript levels. Of these, 39% reflected changes in whole gene expression and 55% reflected transcript isoform changes such as splicing variants (exon skipping, alternative splice site use, intron retention), differential 5¢ UTR (initiation of transcription) use, and differential 3¢ UTR (alternative polyadenylation) use. These results demonstrate that the regulatory effects of genetic variation in a normal human population are far more complex than previously observed. This extra layer of molecular diversity may account for natural phenotypic variation and disease susceptibility. Alternative pre-mRNA processing increases the complexity of eukary￾otic transcriptomes, allowing multiple transcripts and protein iso￾forms with distinct functions to be produced from a single genomic locus1. Within an organism, tissue specific gene isoforms are known to have important functions in development and proper functioning of diverse cell types2. Across individuals, changes in normal isoform structure have phenotypic consequences and have been associated with disease3,4. Splicing defects in a number of genes, such as the cystic fibrosis transmembrane conductance regulator, CFTR, result in several known mendelian disorders5. More subtle changes, such as alternative 3¢ processing and polyadenylation, have recently been associated with complex disorders: OAS1 in severe acute respiratory syndrome6, TAP2 in type I diabetes7, and IRF5 in susceptibility to systemic lupus erythematosus8,9. Several recent studies have suggested that natural variation at the level of whole-gene expression is common in humans and is associated with genetic variants, such as SNPs or copy number variants (CNVs)10–13. Studying variation in gene expression is becoming increasingly important because of its contribution to phenotypic differences among individuals and its possible regulatory and functional relationships to diseases. However, little is known at present about the genetic variation at the sub-transcript level or about differences in multiple transcript isoforms of the same gene. Here, we interrogated transcripts across their entire length, using the Affymetrix GeneChip Human Exon 1.0 ST Array, which can detect splicing differences between various types of samples14–16. Exons within a gene are represented on the microarray by indivi￾dual probe sets, and were considered discrete units for our analysis of transcript isoform-processing differences. We used triplicate samples of lymphoblastoid cell lines (LCLs) derived from 57 unrelated Centre d’Etudes du Polymorphisme Humain (CEPH) CEU individuals (Utah residents with northern and western European ancestry) genotyped by the HapMap consortium17, allowing us to establish a possible genetic basis for any observed variations in transcript isoforms with associated SNPs. A linear regression analysis under a codominant model was carried out to associate probe set expression intensities with the genotypes of all SNP markers within a window of 50 kb flanking the boundaries of the transcript cluster (meta–probe set) containing the probe set. We assessed the statistical significance of the variation using the t-statistic, and used the regression equation to estimate the fold change in expression between the two homozygous genotypes. We used permutation testing18 to determine empirical P-values corre￾sponding to the asymptotic P-values obtained from the regression. Subsequently, we applied the false discovery rate (FDR) correction to establish a cutoff P-value of 9.73
109 , corresponding to the 0.05 FDR level (see Methods). This yielded 757 unique probe sets showing significant SNP associations, belonging to 317 unique meta–probe sets (Supplementary Table 1 online). Although the most significant SNPs may not be the causative polymorphisms responsible for these differences in probe set expression, they are very probably in linkage disequilibrium with the causative polymorphism(s). This is reflected in the distance distribution of associated polymorphisms, most of which are in close proximity to the probe sets (Supplementary Fig. 1 online). The association analysis at the transcript (meta–probe set) level resulted in a 0.05 FDR cutoff of 6.02
107, yielding 127 unique transcripts with significant genetic association at the gene expression Received 9 July 2007; accepted 31 October 2007; published online 13 January 2008; doi:10.1038/ng.2007.57 1Department of Human Genetics, McGill University and 2McGill University and Ge´nome Que´bec Innovation Centre, 740 Dr. Penfield, Room 7210, Montre´al, Que´bec H3A 1A4, Canada. 3Division of Hematology-Oncology, Research Centre, Sainte-Justine Hospital, Montre´al, Que´bec H3T 1C5, Canada. 4Ontario Institute for Cancer Research, MaRS Centre, South Tower, 101 College Street, Suite 800, Toronto, Ontario M5G 1L7, Canada. Correspondence should be addressed to J.M. (jacek.majewski@mcgill.ca). NATURE GENETICS VOLUME 40 [ NUMBER 2 [ FEBRUARY 2008 225 LETTERS © 2008 Nature Publishing Group http://www.nature.com/naturegenetics

LETTERS PS 3527423 versus rs4981998 b 1400 世”日”…想, 〓〓 NM005484 a Figure 1 Analysis steps from identification of significant probe set in the PARP2 gene to idation. (a)Linear regression analysis of expression scores for probe set (PS)3527423 with otypes of SNP rs4981998, giving a P-value of 2.81 x 10-30. Probe set scores for eack individual are shown in red and regression line is indicated w probe set 3527423 in the context of all other probe sets belonging to the same transcript (meta-probe set 3527418) For each probe set, the significance level (P-value)is graphed (red line), along with fold change expression between the mean scores of the two homozygous genotypes(mean /meancc) (vertical blue bars). The solid horizontal red and blue lines represent the and fold change expression for the regression analysis at the meta- be set level against SNP rs4981998. Arrow, probe set 3527423. (c)RT- idation of probe set 352742 flanking exon-body primers e splice site use resulting in a larger second eo? ow the exons. The significant probe set 3527423 is highlighted in red and corresponds to alternat/e.orms 9 Individuals are highlighted by color according to their genotype for SNPrs4s E of parp2 with exon array probe sets for NM 005484 o level. Of these 127 transcripts, all but seven were common to the 317 We proceeded, using two different methods, to validate 32 of our top transcripts derived from the regression analysis at the probe-set level; candidate events distributed among the coding(16),5 UTR (6), and herefore, our final dataset comprised 324 transcripts predicted to 3 UTR(10)regions. For alternative splicing events of internally located ave expression changes at the meta-probe set and/or pro performed RT-PCR We examined the 324 transcripts in greater detail(Fig. 1; examples using exon-body primers in the two exons flanking the candidate in Fig. 2)to determine the nature of the isoform changes on a probe set(Fig. lc). We confirmed 15 probe sets showing SNP transcript level(summarized in Supplementary Table 2 and Supple- association to splicing of a cassette exon or intron(Table 1)and ntary Fig. 2 online). Expression changes were automatically classified them as follows: eight probe sets corresponded to splicing of a classified on the basis of the positions of the variable probe sets, coding exon, four probe sets were located in the 5 UTR and resulted transcript( Supplementary Fig. 2). A large number of genes(127, or use, two probe sets were found within intronic regions and resulted in 39%)showed whole-gene expression changes. However, an even larger intron retention, and the remaining probe set was located in the proportion(55%)of genes showed transcript-isoform changes only, 3 UTR and altered its length. The second, more sensitive validation without an accompanying change in the expression of the entire locus. method using quantitative real-time RT-PCR was applied to differen Nearly half of these transcript variations were at the splicing level (85, tially expressed probe sets within the 5 or 3 UTR and to those or 26%), with the remaining changes at the level of transcript which one of the flanking probe sets was missing in one of the termination (57, or 18%)and initiation(35, or 11%)(Fig. 3). It alternative isoforms. We designed sets of primers to amplify the should be noted that some of the genes showing changes in the differentially expressed probe set itself and compared the resulting expression level of the whole gene also showed further changes in PCR products to ones corresponding to adjacent probe sets showing splicing, transcript termination and/or transcript initiation, suggesting no association to the SNP and also expected to have similar expression that transcript isoform variation constitutes a large part of the genetic levels across all cell lines. Quantitative PCr data was used to perform a variation we have observed. A small number(20, or 6%)of genes linear regression fit with the original associated SNP and confirm the showed very complex patterns of isoform variation that were difficult significance and direction of the association analysis with the micro- to interpret. Notably, when we compare the proportion(18%)of array data at a nominal P-value of 0.05/, where N is the number of significant probe sets within the 3'untranslated regions(UTRs) with candidates tested in the real-time RT-PCR. Using this method,we the proportion of all 3 UTR core probe sets(13%)on the array, we validated six UTR-located probe sets showing SNP association: four found a significant over-representation(Pearsons chi-squared test, the 3 UTR (alternative polyadenylation) and two in the 5 UTR P=5.73x 10-6)of probe sets in this region, indicating that (differential transcriptional initiation). We also used this method on transcript termination variations may occur more frequently than the candidate probe sets that failed our initial validation method owing expected Because predicted changes to the 3'UTR may affect mRNA potentially to low sensitivity of endpoint PCR of minor isoforms, and stability and subcellular localization, this type of isoform variation we were able to validate another four probe sets: two within coding ay have important regulatory roles. These findings illustrate a very regions and two within the 3 UTRs. In total, 25 of 32 candidate probe omplex pattern of expression changes associated with genetic varia- sets were validated, for a success rate of 78%. The remaining 7 probe tion, encompassing alterations at the whole-gene expression level sets failed validation, which can be partially accounted for by unan- andor differences in transcript isofo notated SNPs located within the probe sets possibly leading to altered VOLUME 40 NUMBER 2 FEBRUARY 2008 NATURE GENETICS

level. Of these 127 transcripts, all but seven were common to the 317 transcripts derived from the regression analysis at the probe-set level; therefore, our final dataset comprised 324 transcripts predicted to have expression changes at the meta–probe set and/or probe set level. We examined the 324 transcripts in greater detail (Fig. 1; examples in Fig. 2) to determine the nature of the isoform changes on a transcript level (summarized in Supplementary Table 2 and Supple￾mentary Fig. 2 online). Expression changes were automatically classified on the basis of the positions of the variable probe sets, followed by manual curation based on visualization of the entire transcript (Supplementary Fig. 2). A large number of genes (127, or 39%) showed whole-gene expression changes. However, an even larger proportion (55%) of genes showed transcript-isoform changes only, without an accompanying change in the expression of the entire locus. Nearly half of these transcript variations were at the splicing level (85, or 26%), with the remaining changes at the level of transcript termination (57, or 18%) and initiation (35, or 11%) (Fig. 3). It should be noted that some of the genes showing changes in the expression level of the whole gene also showed further changes in splicing, transcript termination and/or transcript initiation, suggesting that transcript isoform variation constitutes a large part of the genetic variation we have observed. A small number (20, or 6%) of genes showed very complex patterns of isoform variation that were difficult to interpret. Notably, when we compare the proportion (18%) of significant probe sets within the 3¢ untranslated regions (UTRs) with the proportion of all 3¢ UTR core probe sets (13%) on the array, we found a significant over-representation (Pearson’s chi-squared test, P ¼ 5.73
106 ) of probe sets in this region, indicating that transcript termination variations may occur more frequently than expected. Because predicted changes to the 3¢ UTR may affect mRNA stability and subcellular localization, this type of isoform variation may have important regulatory roles. These findings illustrate a very complex pattern of expression changes associated with genetic varia￾tion, encompassing alterations at the whole-gene expression level and/or differences in transcript isoforms. We proceeded, using two different methods, to validate 32 of our top candidate events distributed among the coding (16), 5¢ UTR (6), and 3¢ UTR (10) regions. For alternative splicing events of internally located probe sets, we performed RT-PCR on our entire panel of cell lines using exon-body primers in the two exons flanking the candidate probe set (Fig. 1c). We confirmed 15 probe sets showing SNP association to splicing of a cassette exon or intron (Table 1) and classified them as follows: eight probe sets corresponded to splicing of a coding exon, four probe sets were located in the 5¢ UTR and resulted in the removal of potential promoter sequences or alternative start codon use, two probe sets were found within intronic regions and resulted in intron retention, and the remaining probe set was located in the 3¢ UTR and altered its length. The second, more sensitive validation method using quantitative real-time RT-PCR was applied to differen￾tially expressed probe sets within the 5¢ or 3¢ UTR and to those in which one of the flanking probe sets was missing in one of the alternative isoforms. We designed sets of primers to amplify the differentially expressed probe set itself and compared the resulting PCR products to ones corresponding to adjacent probe sets showing no association to the SNP and also expected to have similar expression levels across all cell lines. Quantitative PCR data was used to perform a linear regression fit with the original associated SNP and confirm the significance and direction of the association analysis with the micro￾array data at a nominal P-value of 0.05/N, where N is the number of candidates tested in the real-time RT-PCR. Using this method, we validated six UTR-located probe sets showing SNP association: four in the 3¢ UTR (alternative polyadenylation) and two in the 5¢ UTR (differential transcriptional initiation). We also used this method on the candidate probe sets that failed our initial validation method owing potentially to low sensitivity of endpoint PCR of minor isoforms, and we were able to validate another four probe sets: two within coding regions and two within the 3¢ UTRs. In total, 25 of 32 candidate probe sets were validated, for a success rate of 78%. The remaining 7 probe sets failed validation, which can be partially accounted for by unan￾notated SNPs located within the probe sets possibly leading to altered PS 3527423 versus rs4981998 MPS 3527418 versus rs4981998 1,400 1,200 1,000 800 600 Probe-set expression 400 200 CC CT Genotype TT P = 2.81 × 10–30 PS 3527423 30 25 20 15 10 5 0 –log10(P-value) 3527419 3527421 3527422 3527423 3527425 NM_005484 NM_001042618 3527419 06994 06993 07357 12145 07056 12057 11882 12812 07022 12763 12043 12760 07055 12814 12144 12813 07034 12872 11881 12815 06985 12874 12146 12873 12891 11994 12239 11830 07345 11832 07000 12762 12154 12761 12155 12892 11993 12044 12249 12248 11995 12264 11992 12156 11840 11839 12234 11829 11831 12750 12751 12003 12004 12005 12006 3527421 3527422 3527423 3527425 3527426 3527427 3527430 3527431 3527432 3527433 3527435 3527439 3527440 3527441 3527446 3527448 3527450 3527452 3527453 3527454 5′ Probe set 0.0 0.5 1.0 log2(fold change) 1.5 2.0 2.5 3.0 0.4 0.3 0.2 ab c d 0.4 0.3 0.2 0.4 0.3 0.2 Figure 1 Analysis steps from identification of significant probe set in the PARP2 gene to validation. (a) Linear regression analysis of expression scores for probe set (PS) 3527423 with genotypes of SNP rs4981998, giving a P-value of 2.81
1030. Probe set scores for each individual are shown in red and regression line is indicated with blue dashes. (b) Visualization of probe set 3527423 in the context of all other probe sets belonging to the same transcript (meta–probe set 3527418). For each probe set, the significance level (P-value) is graphed (red line), along with fold change expression between the mean scores of the two homozygous genotypes (meanTT / meanCC) (vertical blue bars). The solid horizontal red and blue lines represent the significance and fold change expression for the regression analysis at the meta– probe set level against SNP rs4981998. Arrow, probe set 3527423. (c) RT-PCR validation of probe set 3527423 using flanking exon-body primers. Individuals are highlighted by color according to their genotype for SNP rs4981998: CC (red), CT (black), TT (blue). (d) Schematic of 5¢ end of two isoforms of PARP2 with exon array probe sets shown below the exons. The significant probe set 3527423 is highlighted in red and corresponds to alternative 5¢ splice site use resulting in a larger second exon for NM_005484. 226 VOLUME 40 [ NUMBER 2 [ FEBRUARY 2008 NATURE GENETICS LETTERS © 2008 Nature Publishing Group http://www.nature.com/naturegenetics

LETTERS ERAP2 ERAP1 2 Examples of differer oform events observed. Data is graphed as in Figure 1b. (a)Gene expression level changes of assette exon. (b)Differential 3 UTR change of ERAPI resulting in long and short isoforms with alternative stop codon use. (c) Expression of tw Pu叫 TCL6 transcript isoforms that contain different 5 and 3'ends. (d)Increasing significance and fold hange in expression levels toward the 3 end of he CCT2 gene, suggesting genetic variation associated with mRNA stability 男系己吧器器语导导器寻 88R和NR器888%拓等等等哥 888 388888888888888888888 3, 554 genes 0. Differences in statistical strin- Probe s gency and false discovery rate most likely d CCT explain the higher proportion of SNP tions in their study. However, their set of 10 3, 554 genes was preselected for the most variable expression phenotypes among g original set of >8,000 genes. This restricted set of genes may exclude examples of isoform changes without an accompanying change in whole-gene expression, which we observed in our study. In future expression association studies, comparative meta-analyses across dif- ferent microarray designs may help eliminate platform-specific technical artifacts and allow the elucidation of true isoform and We show that tools such as the exon array, 9 hybridization signals(see Methods ) suboptimal primer design, targeting probes to many regions of the gene, give a more complete o limited sensitivity of our validation methods, and/or noise from the picture of the true complexity of variation in gene expression than 5 microarray. We also validated several differentially spliced exons under previously believed. This variation exists at all levels of transcript 2 a more relaxed stringency below our estimated cutoff, indicating that processing, beginning with initiation of transcription, through pre ao the frequency of genes showing SNP-associated changes is probably mRNA splicing 6,202, to alternative polyadenylation, and it has the a greater than what can be estimated from our current analysis. A recent potential to exert diverse cellular responses and phenotypic effects. o estimate suggests that -21%6 of annotated alternatively spliced genes are associated with SNPs that determine the relative abundances of the alternative transcript isoforms. A recent study used Ilumina arrays to capture gene expression information within the CEU population. The Illumina design, along Expression with many other expression platforms, targets probes to the 3 end of genes and cannot identify specific isoform changes Our present results demonstrate that the nature of the changes is qualitatively different than previously reported for several genes in that study. For example our analysis shows that IRF5, implicated in susceptibility to systemic lupus erythematosus, shows differences in the 3 UTR (Fig. 4), where the A allele of rsl0954213 creates a functional polyadenylation site, shortening its 3 UTR9. This result for IRF5 contrasts the original edicted change at the gene expression level0, I3 and occurs because he Illumina array interrogates IRF5 with a probe in the 3 UTR specific Figure 3 Classification of genes showing expression changes at the exon to the long isoform. Other examples previously classified as expression changes include PTER, which we show to have a variation in the 3 categories depending on the nature of the isoform change occurring expression changes at the whole transcript level (green), transcripti UTR,and CI7orf81(also known as DERP6), which shows alternative initiation changes (yellow), alternative splicing of a cassette exon(blue), splicing of a cassette exon. Another interesting example is ERAP2, transcription termination changes (purple), and complex changes of multipl hich has been reported as having an expression change. Our results event types(red). The percentages shown assume a uniform false-positive onfirm this variation in expression; however, we additionally detect rate for all results To obtain a lower bound for the relative frequency of alternative splice-site use in one of the exons(Fig. 2a). Many platforms isoform variants, we have also recalculated the frequencies of the isoform have been used so far in these population-wide expression analyses, changes(but not whole-gene expression and complex changes)based on our ough there is substantial werlap between the studies, signifi- Thus, we obtained the following ranges for each of the changes: whole gene cant discordance also exists. A recent paper identified 374 gene- expression, 39-44%: initiation, 10-11%; splicing. 24-26%: termination expression phenotypes associated with SNP markers from a study of 16-18%; and complex events, 6-7% NATURE GENETICS VOLUME 40 NUMBER 2 I FEBRUARY 2008

hybridization signals19 (see Methods), suboptimal primer design, limited sensitivity of our validation methods, and/or noise from the microarray. We also validated several differentially spliced exons under a more relaxed stringency below our estimated cutoff, indicating that the frequency of genes showing SNP-associated changes is probably greater than what can be estimated from our current analysis. A recent estimate suggests that B21% of annotated alternatively spliced genes are associated with SNPs that determine the relative abundances of the alternative transcript isoforms20. A recent study used Illumina arrays to capture gene expression information within the CEU population13. The Illumina design, along with many other expression platforms, targets probes to the 3¢ end of genes and cannot identify specific isoform changes. Our present results demonstrate that the nature of the changes is qualitatively different than previously reported for several genes in that study. For example, our analysis shows that IRF5, implicated in susceptibility to systemic lupus erythematosus, shows differences in the 3¢ UTR (Fig. 4), where the A allele of rs10954213 creates a functional polyadenylation site, shortening its 3¢ UTR8,9. This result for IRF5 contrasts the original predicted change at the gene expression level10,13 and occurs because the Illumina array interrogates IRF5 with a probe in the 3¢ UTR specific to the long isoform. Other examples previously classified as expression changes include PTER, which we show to have a variation in the 3¢ UTR, and C17orf81 (also known as DERP6), which shows alternative splicing of a cassette exon. Another interesting example is ERAP2, which has been reported as having an expression change10. Our results confirm this variation in expression; however, we additionally detect alternative splice-site use in one of the exons (Fig. 2a). Many platforms have been used so far in these population-wide expression analyses, and although there is substantial overlap between the studies, signifi- cant discordance also exists. A recent paper identified 374 gene￾expression phenotypes associated with SNP markers from a study of 3,554 genes10. Differences in statistical strin￾gency and false discovery rate most likely explain the higher proportion of SNP associa￾tions in their study. However, their set of 3,554 genes was preselected for the most variable expression phenotypes among an original set of 48,000 genes. This restricted set of genes may exclude examples of isoform changes without an accompanying change in whole-gene expression, which we observed in our study. In future expression association studies, comparative meta-analyses across dif￾ferent microarray designs may help eliminate platform-specific technical artifacts and allow the elucidation of true isoform and gene-level variations. We show that tools such as the exon array, targeting probes to many regions of the gene, give a more complete picture of the true complexity of variation in gene expression than previously believed. This variation exists at all levels of transcript processing, beginning with initiation of transcription, through pre￾mRNA splicing16,20,21, to alternative polyadenylation, and it has the potential to exert diverse cellular responses and phenotypic effects. 20 18 16 14 12 –log10 (P-value) 2821369 2821370 2821371 2821372 2821373 2821374 2821378 2821379 2821382 2821383 2821385 2821387 2821389 2821390 2821392 2821394 2821399 2821400 2821402 2821403 2821404 2821406 2821408 2821409 Probe set ERAP2 log2 (fold change) –2 –1 0 1 a b d log2 (fold change) 15 10 –log 5 10 (P-value) 3421631 3421634 3421635 3421636 3421637 3421638 3421640 3421643 3421644 3421645 3421650 3421652 3421653 3421654 3421655 3421656 3421657 3421658 3421661 3421666 3421667 3421668 Probe set 0.5 1.0 1.5 0 2.0 0.0 25 20 15 10 5 0 –log10 (P-value) 2868182 2868181 2868180 2868179 2868178 2868177 2868176 2868173 2868172 2868170 2868169 2868168 2868167 2868161 2868160 2868157 2868156 2868155 2868154 2868152 2868151 2868146 2868145 2868144 2868143 2868142 2868141 2868134 2868133 Probe set ERAP1 CCT2 log2 (fold change) –1.0 –0.5 0.5 0.0 1.5 1.0 c 10 8 6 4 2 –log10 (P-value) 3550142 3550143 3550144 3550145 3550152 3550153 3550154 3550155 3550156 3550157 3550159 3550160 3550161 3550162 3550163 3550164 3550165 3550166 3550167 3550169 3550170 3550174 3550176 3550177 3550178 3550181 3550182 3550183 3550184 3550185 Probe set TCL6 log2 (fold change) –2 –1 0 1 0 2 –3 Expression 39% Initiation 11% Splicing 26% Termination 18% Complex 6% Figure 3 Classification of genes showing expression changes at the exon and/or transcript level. The 324 genes were classified into separate categories depending on the nature of the isoform change occurring: expression changes at the whole transcript level (green), transcription initiation changes (yellow), alternative splicing of a cassette exon (blue), transcription termination changes (purple), and complex changes of multiple event types (red). The percentages shown assume a uniform false-positive rate for all results. To obtain a lower bound for the relative frequency of isoform variants, we have also recalculated the frequencies of the isoform changes (but not whole-gene expression and complex changes) based on our current false positive rate estimate of B20% (from validation experiments). Thus, we obtained the following ranges for each of the changes: whole gene expression, 39–44%; initiation, 10–11%; splicing, 24–26%; termination, 16–18%; and complex events, 6–7%. Figure 2 Examples of different types of transcript isoform events observed. Data is graphed as in Figure 1b. (a) Gene expression level changes of ERAP2, including alternative splicing of a cassette exon. (b) Differential 3¢ UTR change of ERAP1 resulting in long and short isoforms with alternative stop codon use. (c) Expression of two TCL6 transcript isoforms that contain different 5¢ and 3¢ ends. (d) Increasing significance and fold change in expression levels toward the 3¢ end of the CCT2 gene, suggesting genetic variation associated with mRNA stability. NATURE GENETICS VOLUME 40 [ NUMBER 2 [ FEBRUARY 2008 227 LETTERS © 2008 Nature Publishing Group http://www.nature.com/naturegenetics

LETTERS Table l Validation of candidate probe sets Gene chromosomal location Type of event evidence CEPI 3.71×1019ch18:1304770-13048132 Coding ZNF83 2.72×10-10chr19:57808794-57808830 Intron retention C1Torf57 3724617rs37603725.54×1012chr17:42793744-42793848 Exon skipping CAST 821249r77247597.17×10 chr5:96102207-96102239 Exon skipp CD46 377476rs48443901.06×1014chr1:204329527-204329556odng Exon skipping ATPSSL 10434139.38×101lchr19:46631033-46631167 Exon skipping ERAP2 rs22555468.37×1022chr596261677-96261705 Alternative splice site use 2 POMZP3 20053543.77×1022ch7:75892151-75892256 Exon skipping 2670619 17170205.99×1 chr3:419324784193251 s PARP2 3527423s2297616281×10-37chr14:19883099-19883123 Coding Alternative splice site ATPIF 232738352481974426×10-1lch1:28248451-28248478 Coding Alternative splice site E MRPL4 3303658 rs12241232 1.24 x 1011 chr10: 102731257-102731290 Coding Exon skipping, differential stop 451M21192588913rs109307851.93×10-28ch2:178022380-1780224825UTR Exon skipping 3358076r5118213924.34×10-15 chr11:494826494888 Exon skipping SNAIL 3725089rs72240144.20×10chr17:43543086435431165UTR Exon skipping USMG5 2.66×10-24chr10:105143981-1051440955′UTR Exon skipping SEPI5 1013chr1:87091818-87092018 Differential 5 UTR length 2.12×10-10 chr6: 8380460-8380572 5 UTR Differential 5 UTR length 666666 rs25219852.55×1 chr17:7100907-7100934 3’UT Exon skipping, differential ERAPI 2868133s77058276.09×1019chr5:96123330-961244833 UTR Differential3' UTR length 2950168rs3763355198×1013chr6:32897620-328978803 UTR Alternative splice site us differential 023264rs69699308.27×1022chr7: 12-1281837233UTR ifferential 3" UTR length 59990981.46×1012ch 3236819rs1055340525×10-18ch10:16595519-165956413UTR Differential 3 UTR length 2430765s13259333.53×108chr:119285989-1192862363 UTR Differentia3 UTR length andidate probe sets validated by qualitative or quantitative RT. ated along with the sNP and P- analysis. The chromosomal lacation of the probe set is also shown, includin tive location within the gene. The nature of the isoform change is indicated, as is any existing Ref Seq or EST evidence of this change. Transcript alterations within coding regions of the gene, such as the SNPs resulting in these changes will lead to the possible identification addition or removal of sequences coding for functional domains or of new regulatory motifs and is currently being undertaken he introduction of premature stop codons, may greatly alter the Earlier studies suggested that gene expression constituted ar protein sequence, structure and function-,. Changes outside the important piece of human variation, and although it remains a coding regions can also have wide-ranging regulatory consequences. significant aspect, the added complexity of transcript-processin Differential exon selection within the 5 and 3" UTRs may alter mRNA variations and the potential outcome of these differences greatly stability and translational efficiency by the addition or removal of alter our earlier perceptions. We estimate that between 50 and 55% gulatory sequences. In some genes( for example, ATPIFI and TAP2 ), of gene expression variation is isoform based. Our results constitute an lection of an alternative splice site for the terminal exon resulted in important change in way we view the effects of common genetic differential stop codon use and, consequently, changes in the length variation in humans and highlight the need for broader investigation and composition of the 3'UTR. Alterations in the 3"UTR can also be into the causes of differential gene expression, as well as previously effected by alternative use of polyadeny lation sites, and approximately found and new disease associations that lack clear functional variants half of human genes are predicted to contain several polyadenylation sites, resulting in transcripts with different 3' UTR lengths24,2. METHODS Altering a functional polyadenylation site through a single poly- Cell line preparation. We obtained triplicate RNA samples from LCLs derived morphism may lead to isoform switching. The 3"UTR is also involved from the parents of 30 CEPH(CEU) trios(60 indivi had been in post-transcriptional regulation through the targeting of specific genotyped for approximately 4 million SNPs by the al HapMap UTR sequences by microRNAs(miRNA26,27. Expression of multiple Project". Cells were grown at 37C and 5%COz in RPMI 1640 medium isoforms may be indirectly controlled through the differential expres- (Invitrogen) supplemented with 15%(vol/vol)heat-inactivated FCS(Sigma sion of miRNAs or by polymorphisms in these miRNA-specific Aldrich), 2 mMi-glutamine(Invitrogen) and penicilin/strept equences. The end consequence of many of these alterations in the collected at a density of c.8×10°tol.l×10°cels/ ml. Cells were then UTRs affects a cascade of downstream processes such as stability, resuspended and lysed in TRIzol reagent (Invitrogen). Three successive growths localization and translation efficiency, and it directly contributes to were performed(corresponding to the second, fourth and sixth passages)after typic diversity and possible disease states. A systematic chara- thawing frozen cell aliquots. Three cell lines showed extremely poor growth and zation of the polymorphisms to determine the true causative were not used in the study, leaving 57 LCLs for subsequent analyses VOLUME 40 NUMBER 2 FEBRUARY 2008 NATURE GENETICS

Transcript alterations within coding regions of the gene, such as the addition or removal of sequences coding for functional domains or the introduction of premature stop codons, may greatly alter the protein sequence, structure and function22,23. Changes outside the coding regions can also have wide-ranging regulatory consequences. Differential exon selection within the 5¢ and 3¢ UTRs may alter mRNA stability and translational efficiency by the addition or removal of regulatory sequences. In some genes (for example, ATPIF1 and TAP2), selection of an alternative splice site for the terminal exon resulted in differential stop codon use and, consequently, changes in the length and composition of the 3¢ UTR. Alterations in the 3¢ UTR can also be effected by alternative use of polyadenylation sites, and approximately half of human genes are predicted to contain several polyadenylation sites, resulting in transcripts with different 3¢ UTR lengths24,25. Altering a functional polyadenylation site through a single poly￾morphism may lead to isoform switching. The 3¢ UTR is also involved in post-transcriptional regulation through the targeting of specific UTR sequences by microRNAs (miRNA)26,27. Expression of multiple isoforms may be indirectly controlled through the differential expres￾sion of miRNAs or by polymorphisms in these miRNA-specific sequences. The end consequence of many of these alterations in the UTRs affects a cascade of downstream processes such as stability, localization and translation efficiency, and it directly contributes to phenotypic diversity and possible disease states. A systematic chara￾cterization of the polymorphisms to determine the true causative SNPs resulting in these changes will lead to the possible identification of new regulatory motifs and is currently being undertaken. Earlier studies suggested that gene expression constituted an important piece of human variation, and although it remains a significant aspect, the added complexity of transcript-processing variations and the potential outcome of these differences greatly alter our earlier perceptions. We estimate that between 50 and 55% of gene expression variation is isoform based. Our results constitute an important change in way we view the effects of common genetic variation in humans and highlight the need for broader investigation into the causes of differential gene expression, as well as previously found and new disease associations that lack clear functional variants. METHODS Cell line preparation. We obtained triplicate RNA samples from LCLs derived from the parents of 30 CEPH (CEU) trios (60 individuals) that had been genotyped for approximately 4 million SNPs by the International HapMap Project17. Cells were grown at 37 1C and 5% CO2 in RPMI 1640 medium (Invitrogen) supplemented with 15% (vol/vol) heat-inactivated FCS (Sigma￾Aldrich), 2 mM L-glutamine (Invitrogen) and penicillin/streptomycin (Invitro￾gen). Cell growth was monitored with a hemocytometer and cells were collected at a density of 0.8
106 to 1.1
106 cells/ml. Cells were then resuspended and lysed in TRIzol reagent (Invitrogen). Three successive growths were performed (corresponding to the second, fourth and sixth passages) after thawing frozen cell aliquots. Three cell lines showed extremely poor growth and were not used in the study, leaving 57 LCLs for subsequent analyses. Table 1 Validation of candidate probe sets Gene Probe set SNP P-value Chromosomal location Probe set location Type of event RefSeq/EST evidence CEP192 3779862 rs482360 3.71
10–19 chr18:13047770–13048132 Coding Intron retention Yes ZNF83 3869658 rs1012531 2.72
10–10 chr19:57808794–57808830 Coding Intron retention Yes C17orf57 3724617 rs3760372 5.54
10–12 chr17:42793744–42793848 Coding Exon skipping Yes CAST 2821249 rs7724759 7.17
10–16 chr5:96102207–96102239 Coding Exon skipping Yes CD46 2377476 rs4844390 1.06
10–14 chr1:204329527–204329556 Coding Exon skipping Yes ATP5SL 3863093 rs1043413 9.38
10–11 chr19:46631033–46631167 Coding Exon skipping Yes ERAP2 2821389 rs2255546 8.37
10–22 chr5:96261677–96261705 Coding Alternative splice site use Yes POMZP3 3057764 rs2005354 3.77
10–22 chr7:75892151–75892256 Coding Exon skipping Yes ULK4 2670619 rs1717020 5.99
10–11 chr3:41932478–41932514 Coding Exon skipping No PARP2 3527423 rs2297616 2.81
10–37 chr14:19883099–19883123 Coding Alternative splice site use Yes ATPIF1 2327383 rs2481974 4.26
10–11 chr1:28248451–28248478 Coding Alternative splice site use Yes MRPL43 3303658 rs12241232 1.24
10–11 chr10:102731257–102731290 Coding Exon skipping, differential stop codon use and 3¢ UTR length Yes DKFZp451M2119 2588913 rs10930785 1.93
10–28 chr2:178022380–178022482 5¢ UTR Exon skipping Yes RNH1 3358076 rs11821392 4.34
10–15 chr11:494826–494888 5¢ UTR Exon skipping Yes SNX11 3725089 rs7224014 4.20
10–9 chr17:43543086–43543116 5¢ UTR Exon skipping Yes USMG5 3304753 rs7911488 2.66
10–24 chr10:105143981–105144095 5¢ UTR Exon skipping Yes SEP15 2421300 rs1407131 7.57
10–13 chr1:87091818–87092018 5¢ UTR Differential 5¢ UTR length Yes SLC35B3 2941033 rs3799255 2.12
10–10 chr6:8380460–8380572 5¢ UTR Differential 5¢ UTR length Yes C17orf81 3708382 rs2521985 2.55
10–13 chr17:7100907–7100934 3¢ UTR Exon skipping, differential 3¢ UTR length Yes ERAP1 2868133 rs7705827 6.09
10–19 chr5:96123330–96124483 3¢ UTR Differential 3¢ UTR length Yes TAP2 2950168 rs3763355 1.98
10–13 chr6:32897620–32897880 3¢ UTR Alternative splice site use, differential 3¢ UTR length Yes IRF5 3023264 rs6969930 8.27
10–22 chr7:128183412–128183723 3¢ UTR Differential 3¢ UTR length Yes PPIL2 3938301 rs5999098 1.46
10–12 chr22:20374916–20375108 3¢ UTR Differential 3¢ UTR length Yes PTER 3236819 rs1055340 5.25
10–18 chr10:16595519–16595641 3¢ UTR Differential 3¢ UTR length No WARS2 2430765 rs1325933 3.53
10–8 chr1:119285989–119286236 3¢ UTR Differential 3¢ UTR length Yes List of candidate probe sets validated by qualitative or quantitative RT-PCR. The gene name and the significant probe set are indicated along with the SNP and P-value from the linear regression analysis. The chromosomal location of the probe set is also shown, including its relative location within the gene. The nature of the isoform change is indicated, as is any existing RefSeq or EST evidence of this change. 228 VOLUME 40 [ NUMBER 2 [ FEBRUARY 2008 NATURE GENETICS LETTERS © 2008 Nature Publishing Group http://www.nature.com/naturegenetics

LETTERS rs10954213 Quantitative RT-PCR validation Ps3023263 P=0.1 Short isoform b Ps3023264 250=0.33 P=827×10n2 2500 2000 Ps3023264 25P=104×10 Genotype E Figure 4 Validation of 3 UTR change in IRF5 by quantitative real-time RT-PCR. (a)Schematic of the 3 ends of the long and short isoforms of /RF5 Exons are shown in blue introns are dashed lines d solid horizontal lines below the exons indicate probe sets. b)Regression analyses of probe sets 0 3023263 and 3023264 against SNP rs10954213. (c)Regression analysis of Ct counts fro quantitative real-time RT-PCR against the ge of SNP rs10954213, to confirm the original microarray data. We used two sets of primers on the panel of individuals, designed to amplify probe sets 3023263 and 3023264, respectively. Q. Affymetrix exon arrays. We isolated RNA using TRIzol reagent following the probe intensity levels by magnitude of response, removin s manufacturer's instructions(Invitrogen) and assessed the RNA quality using seemed to be in the background. Probe intensities were extracted for a series RNA 6000 Nano Chips with the Agilent 2100 Bioanalyzer(Agilent). Biotin- of 16,934 antigenomic probes targeted to nonhuman sequences and averaged z labeled targets for the microarray experiment were prepared using I ug of total by their relative G+C content. The threshold for background expression eo RNA. Ribosomal RNA was removed with the RiboMinus Human/Mouse was defined as the average intensity for a given G+C content plus 2 s.d. s Transcriptome Isolation Kit (Invitrogen) and cDNA was synthesized using For any given genomic probe on the array, if the intensity across all sampl the Gene Chip Wr (Whole Transcript) Sense Target Labeling and Control was below the threshold for the same G+C percentage, then it was consi- Reagents kit as described by the manufacturer(Affymetrix). The sense cDNA dered background and masked from the analysis. In total, 670, 809 probes as then fragmented by uracil DNa glycosylase and apurinic/apyrim corresponding to core annotated probe sets from the analys endonuclease-I and biotin-labeled with terminal deoxynucleotidyl trans- reducing the number of core probe sets in the analysis to 244, 027 probe sets. ferase using the Gene Chip wrTeminal labeling kit (attymetrix). Hybridiza- Association analysis and multiple test correction. We examined probe se tion was performed using 5 micrograms of biotinylated target, which was incubated with the GeneChip Human Exon 1.0 ST array (Affymetrix)at 45 C expression levels for association with flanking SNPs. For each of the 244,027 tested for association of the by washing and specifically bound target was detected using the genechip expression levels to HapMap phase Il (release 2n) SNPs with a minor allele (Affymetrix). The arrays were scanned using the Gene Chip Scanner 3000 7G containing the probe set, using a linear regression model in the R software (Affymetrix)and raw data was extracted from the scanned images and analyzed package. Raw P-values were obtained from the regression using the standard ith the Affymetrix Power Tools software package(Affymetrix) To correct for testing of associations between multiple probe sets and SNPs, Preprocessing and analysis of array hybridization data. The Affymetrix we carried out permutation tests followed by FDR correction. within each Power Tools software package was used to quantile-normalize the probe expression-versus-genotype matrix, we randomly permuted the expression fluorescence intensities and to summarize the probe set (representing exon values for all probe sets belonging to the same meta-probe set(to prese expression) and meta-probe set(representing gene expression) intensities using the haplotype block structure). For each expression measurement, a probe logarithmic-intensity error model(see URLs below). High false- puted and retained only the highest asymptotic P-value and produced the positive rates are common in microarray studies, and previous studies have distribution of maximum P-values within the permuted dataset. The maximum suggested that a major factor arises from probes overlapping SNPs asymptotic P-values from the experimental data were then converted into that result in changes to hybridization intensity potentially influencing the empirical P-values by mapping onto the permuted distribution. The above apparent association between the snP genotype and probe intensities. procedure corrects for testing multiple SNPs against each expression valu reduce potential influences of SNPs on false positives, all probes containing Subsequently, we performed an FDR correction2on the empirical P-values, te known SNPs(dbSNP release 126) were masked out before summarizing control the FDR across multiple expression values. The procedure was applied robe set and meta-probe set scores. The presence of unannotated SNPs separately to measurements at the probe set and meta-probe set levels. We used affecting probe hybridization will remain(see below), but these cannot be a 0.05 FDR criterion as a significance cutoff in our analysis. For the sake of detected by any statistical methods except for the impractical solution of clarity, all of the values and cutoffs quoted in the results correspond to the raw, ng all probes across the panel used in the study. We also filtered uncorrected P-values. NATURE GENETICS VOLUME 40 NUMBER 2 I FEBRUARY 2008

Affymetrix exon arrays. We isolated RNA using TRIzol reagent following the manufacturer’s instructions (Invitrogen) and assessed the RNA quality using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent). Biotin￾labeled targets for the microarray experiment were prepared using 1 mg of total RNA. Ribosomal RNA was removed with the RiboMinus Human/Mouse Transcriptome Isolation Kit (Invitrogen) and cDNA was synthesized using the GeneChip WT (Whole Transcript) Sense Target Labeling and Control Reagents kit as described by the manufacturer (Affymetrix). The sense cDNA was then fragmented by uracil DNA glycosylase and apurinic/apyrimidic endonuclease-1 and biotin-labeled with terminal deoxynucleotidyl trans￾ferase using the GeneChip WT Terminal labeling kit (Affymetrix). Hybridiza￾tion was performed using 5 micrograms of biotinylated target, which was incubated with the GeneChip Human Exon 1.0 ST array (Affymetrix) at 45 1C for 16–20 h. After hybridization, nonspecifically bound material was removed by washing and specifically bound target was detected using the GeneChip Hybridization, Wash and Stain kit, and the GeneChip Fluidics Station 450 (Affymetrix). The arrays were scanned using the GeneChip Scanner 3000 7G (Affymetrix) and raw data was extracted from the scanned images and analyzed with the Affymetrix Power Tools software package (Affymetrix). Preprocessing and analysis of array hybridization data. The Affymetrix Power Tools software package was used to quantile-normalize the probe fluorescence intensities and to summarize the probe set (representing exon expression) and meta–probe set (representing gene expression) intensities using a probe logarithmic-intensity error model (see URLs below). High false￾positive rates are common in microarray studies, and previous studies have suggested that a major factor arises from probes overlapping SNPs that result in changes to hybridization intensity28, potentially influencing the apparent association between the SNP genotype and probe intensities. To reduce potential influences of SNPs on false positives, all probes containing known SNPs (dbSNP release 126) were masked out before summarizing probe set and meta–probe set scores. The presence of unannotated SNPs affecting probe hybridization will remain (see below), but these cannot be detected by any statistical methods except for the impractical solution of resequencing all probes across the panel used in the study. We also filtered probe intensity levels by magnitude of response, removing probes that seemed to be in the background. Probe intensities were extracted for a series of 16,934 antigenomic probes targeted to nonhuman sequences and averaged by their relative G+C content. The threshold for background expression was defined as the average intensity for a given G+C content plus 2 s.d. For any given genomic probe on the array, if the intensity across all samples was below the threshold for the same G+C percentage, then it was consi￾dered background and masked from the analysis. In total, 670,809 probes corresponding to core annotated probe sets were masked from the analysis, reducing the number of core probe sets in the analysis to 244,027 probe sets. Association analysis and multiple test correction. We examined probe set expression levels for association with flanking SNPs. For each of the 244,027 core probe sets and 17,653 meta–probe sets, we tested for association of the expression levels to HapMap phase II (release 21) SNPs with a minor allele frequency of at least 5% within a 50-kb region flanking either side of the gene containing the probe set, using a linear regression model in the R software package. Raw P-values were obtained from the regression using the standard asymptotic t-statistic. To correct for testing of associations between multiple probe sets and SNPs, we carried out permutation tests followed by FDR correction. Within each expression-versus-genotype matrix, we randomly permuted the expression values for all probe sets belonging to the same meta–probe set (to preserve the haplotype block structure). For each expression measurement, we com￾puted and retained only the highest asymptotic P-value and produced the distribution of maximum P-values within the permuted dataset. The maximum asymptotic P-values from the experimental data were then converted into empirical P-values by mapping onto the permuted distribution. The above procedure corrects for testing multiple SNPs against each expression value. Subsequently, we performed an FDR correction29 on the empirical P-values, to control the FDR across multiple expression values. The procedure was applied separately to measurements at the probe set and meta–probe set levels. We used a 0.05 FDR criterion as a significance cutoff in our analysis. For the sake of clarity, all of the values and cutoffs quoted in the results correspond to the raw, uncorrected P-values. PS 3023263 2,500 Long isoform Short isoform Probe set 3023261 3023262 A G rs10954213 3023263 3023264 2,000 1,500 1,000 500 Probe set intensity 0 AA AG Genotype Microarray data GG P = 0.33 b a c PS 3023264 2,500 2,000 1,500 1,000 500 Probe set intensity 0 AA AG Genotype GG P = 8.27 × 10–22 PS 3023263 25 20 15 10 5 Ct count 0 AA AG Genotype Quantitative RT-PCR validation GG P = 0.11 25 20 15 10 5 Ct count 0 PS 3023264 AA AG Genotype GG P = 1.04 × 10–8 Figure 4 Validation of 3¢ UTR change in IRF5 by quantitative real-time RT-PCR. (a) Schematic of the 3¢ ends of the long and short isoforms of IRF5. Exons are shown in blue, introns are dashed lines, and solid horizontal lines below the exons indicate probe sets. (b) Regression analyses of probe sets 3023263 and 3023264 against SNP rs10954213. (c) Regression analysis of Ct counts from quantitative real-time RT-PCR against the genotype of SNP rs10954213, to confirm the original microarray data. We used two sets of primers on the panel of individuals, designed to amplify probe sets 3023263 and 3023264, respectively. NATURE GENETICS VOLUME 40 [ NUMBER 2 [ FEBRUARY 2008 229 LETTERS © 2008 Nature Publishing Group http://www.nature.com/naturegenetics

LETTERS Classification of transcript isoforms. ed an automated conservatively masked out all probes containing SNPs categorize the transcriptional and isoform The algorithm fi nis problem. However, probes containing unannotated SNPs are anscripts as expression variants if there is an association of the entire meta- not ac for: therefore, we wanted to ne effect of these unknown probe set significant at the P< 6.02 x 10-7 level(see above for explanation of SNPs on our analysis. We selected 83 genes, each of which contained only a the cutoffs). Subsequently, the algorithm identifies all individual probe sets single significant probe set. Many(63)of these probe sets are supported by a ignificant at the P<9. x 10-9 level that do not belong to the expression single independent, nonoverlapping probe, and such probe sets are the most variants detected above. All such significant probe sets are then grouped into susceptible to the effect of SNPs, because every probe could potentially be blocks corresponding to exons, according to their Refseq annotation. Each affected by a single SNP. We sequenced the probe sets from the cell lines of six nificant block is classified as an initiation, splicing or termination change individuals, three from each of the two homozygous genotypes of the ccording to its position within the transcript(3, internal, or 5, respectively). associated SNP. We observed that the sequences for 56 probe sets(67.5%) e classified as complex. Finally, all results were manually curated. To visualize the true events and not an artifact of one or more SNPs located in the individual 2 potential nature of the isoform changes on a gene level, the probe sets were probes representing the probe set. In the remaining 27 probe sets(32.5%),we hanges, we plotted the P-values of all the corresponding probe sets and with one of the two homozygous sample groups, most likely giving rise to the E overlaid the told change expression levels between the two homozygous apparent false-positive hit. We excluded these 27 probe sets from our candidat (Supplementary Fig. 2). We made minor adjustments(23 of 324 events) to by two or more independent probes, and are much less susceptible to the effect he automated classifications, mostly in cases where the designations were not of unknown SNPs. Only 2 out of the 32 candidates from the final dataset consistent with annotated alternative isoform structures or where the Affyme- selected for validation(6%)contained previously unidentified SNPs and hence ix transcript annotation was incorrect. failed validation, showing that the effect of SNPs on the final results presented DNase I (Ambion)for 30 min to remove any remaining genomic Dn. U of Validation of transcript isoform changes. Total RNA was treated with 4 U of URLs. Results from regression analyses at the probe set and meta-probe set strand complementary DNA was synthesized using random hexamers(Invitro. levels, including gene-level plots of expression changes, and ot e gen)and Superscript ll reverse transcriptase(Invitrogen). All primers used for information can be found at the GRiD(Genetic Regulators in Di CRt-pCrreactions(supplementaryTable3online)weredesignedusing(http://www.regulatorygenomics.org).Fortheprobelogarithm Primer3(ref. 30) software. Candidate probe sets showing association w error model ttp//www.affymetrix.com/support/technic 2 validated in two ways, depending on their location within the gene. For all plier_technote-pdf. probe sets located within coding exons and possessing flanking exons in 3 flanking exons. Approximately 20 ng of total cDNA was then amplified by PCr nin the adjacent Expression Omnibus: The data discussed in this publication are accessible through the gEo Series accession number GSE9372. g using Hot Start Taq Polymerase(Qiagen) with an activation step at 95 C 15 min)followed by 35 cycles at 95C(30 s), 58C(30 s)and 72"C(40 s)and Note: Supplementary information is available on the Nature Genetics website. 重6c包n℃m,Amcm zed by ACKNOWLEDGMENTS located within 5 or 3 untranslated regions or within exons The authors would like to thank D Serre, T. Pastinen, E Harmsen and n that did not have a flanking exon, we designed a set of primers to amplify the H. Zuzan for helpful discussions and D. Sinnett for technical assistance o differentially expressed candidate probe set itself. For comparison, other prime pairs were designed to amplify products that corresponded to the adjacent Canadian Institutes of Health Research(CIHR).TLH. is the recipient of probe sets and Clinician-Scientist Award in Translational Research by the Burroughs ot significantly associated with the same SNP. Total Wellcome Fund and an Investigator Award from CIHR J.M. is a recipient pression measurements were carried out using real-time PCR with Power of a Canada research chair turer'sinstructiononanAbI7900ht(apPliEDBiosystems)instrumentTHePubishedonlineathtp/lwtnatresatlregenoniseathtplhpgnature.com/ 8 ng of total cDNA and 0.32 uM of gene-specific primers; cycling, 95C reprintsandpermissions 15 min) and 95C(20 s)58C(30 s),72C(45 s) for 40 cycles. Relative quantification of each amplicon was evaluated on RNA from 57 cell lines in Kim, H, Klein, R, Majewski, J. &Ott, J. Estimating rates of alternative splicing in triplicate. For each amplicon, a standard curve was established using dilution 2. mlamkmabs anMiecvertebrates, Nat. Genet. 36, 915-917(2004) eries of a mix of cDNA samples with known total cDNA concentration. Human Biochem72,291-336(2003) 18S rRNA was also quantified using TaqMan probes as a control for well-to-well 3. Faustino, N.A. Cooper, T.A. Pre-mRNA splicing and human disease. Genes Dev. 17, normalization (Taq Man Pre-Developed Assay Reagents for Gene Expression Nissim-Rafinia, M.& Kerem, B The splicing machinery is a genetic modifier of disease Human 18S rRNA, 4319413E, Applied Biosystems). The cycle threshold(Cr) severity. Trends Genet. 21, 480-483(2005 values for each replicate were transformed to relative concentrations using the 5. Zielenski, J. Genotype and phenotype in cystic fibrosis. Respiration 67, 117-133 stimated standard curve function(SDS 2.1, Applied Biosystems) and normal ed based on 18S real-time data from the same samples to account for well-to- 6. Field, LL. ef al. OASI splice site polymorphism controlling antiviral enzyme well variability. The quantitative data was used in regression analyses with the activity influences susceptibility to type 1 diabetes. Diabetes 54 ne SNP identified in the original association to confirm the significance, using 7. Qu, H.Q. et al. Genetic control of alternative splicing in the TAP2 gene: possible a P-value threshold of 0.05/N where N is the number of candidate genes tested using this method. The regression line was required to be in the same direction 8. Cunninghame Graham, D Association of IRF5 in UK SL es identifies a original association. Quantitative RT-PCR of the control probe sets 9. Graham, R.R. et al. Three functional variants of IFN regulatory factor 5(IRF5)define ng no association with the SNP were also required to be nonsignificant at ive haplotypes for human lupus. Proc. Natl. Acad. Sci. USA 104 10. Cheung, V.G. ef af Mapping determinants of human gene expression by regional and genome-wide association. Nature 437, 1365-1369(2005) Effect of unannotated SNPs on the analysis. We have previously shown that 11. Spielman, R.S. et al. Common genetic variants account for differences in gene NPs located within probes may affect their hybridization to target DNA,and expression among ethnic groups. Nat Genet. 39, 226-231 (2007). VOLUME 40 NUMBER 2 FEBRUARY 2008 NATURE GENETICS

Classification of transcript isoforms. We developed an automated method to categorize the transcriptional and isoform changes. The algorithm first classifies transcripts as expression variants if there is an association of the entire meta– probe set significant at the P o 6.02
107 level (see above for explanation of the cutoffs). Subsequently, the algorithm identifies all individual probe sets significant at the P o 9.73
109 level that do not belong to the expression variants detected above. All such significant probe sets are then grouped into blocks corresponding to exons, according to their RefSeq annotation. Each significant block is classified as an initiation, splicing or termination change according to its position within the transcript (3¢, internal, or 5¢, respectively). Cases with two or more of the above events occurring in a single transcript are classified as complex. Finally, all results were manually curated. To visualize the potential nature of the isoform changes on a gene level, the probe sets were examined in the context of their transcript, mRNA, and EST information. For each gene predicted to have SNP-associated transcript- or exon-level expression changes, we plotted the P-values of all the corresponding probe sets and overlaid the fold change expression levels between the two homozygous genotypes for the significant SNP identified in the association analyses (Supplementary Fig. 2). We made minor adjustments (23 of 324 events) to the automated classifications, mostly in cases where the designations were not consistent with annotated alternative isoform structures or where the Affyme￾trix transcript annotation was incorrect. Validation of transcript isoform changes. Total RNA was treated with 4 U of DNase I (Ambion) for 30 min to remove any remaining genomic DNA. First￾strand complementary DNA was synthesized using random hexamers (Invitro￾gen) and Superscript II reverse transcriptase (Invitrogen). All primers used for RT-PCR reactions (Supplementary Table 3 online) were designed using Primer3 (ref. 30) software. Candidate probe sets showing association were validated in two ways, depending on their location within the gene. For all probe sets located within coding exons and possessing flanking exons in all known RefSeq isoforms, we designed locus-specific primers within the adjacent flanking exons. Approximately 20 ng of total cDNA was then amplified by PCR using Hot Start Taq Polymerase (Qiagen) with an activation step at 95 1C (15 min) followed by 35 cycles at 95 1C (30 s), 58 1C (30 s) and 72 1C (40 s) and a final extension step at 72 1C (5 min). Amplicons were visualized by electrophoresis on a 2.5% agarose gel. For probe sets located within 5¢ or 3¢ untranslated regions or within exons that did not have a flanking exon, we designed a set of primers to amplify the differentially expressed candidate probe set itself. For comparison, other primer pairs were designed to amplify products that corresponded to the adjacent probe sets and were not significantly associated with the same SNP. Total expression measurements were carried out using real-time PCR with Power SYBR Green PCR Master Mix (Applied Biosystems) following the manufac￾turer’s instruction on an ABI 7900HT (Applied Biosystems) instrument. The reaction was set up in 10 ml final volume applying the following conditions: 8 ng of total cDNA and 0.32 mM of gene-specific primers; cycling, 95 1C (15 min) and 95 1C (20 s), 58 1C (30 s), 72 1C (45 s) for 40 cycles. Relative quantification of each amplicon was evaluated on RNA from 57 cell lines in triplicate. For each amplicon, a standard curve was established using dilution series of a mix of cDNA samples with known total cDNA concentration. Human 18S rRNA was also quantified using TaqMan probes as a control for well-to-well normalization (TaqMan Pre-Developed Assay Reagents for Gene Expression – Human 18S rRNA, 4319413E, Applied Biosystems). The cycle threshold (Ct) values for each replicate were transformed to relative concentrations using the estimated standard curve function (SDS 2.1, Applied Biosystems) and normal￾ized based on 18S real-time data from the same samples to account for well-to￾well variability. The quantitative data was used in regression analyses with the same SNP identified in the original association to confirm the significance, using a P-value threshold of 0.05/N where N is the number of candidate genes tested using this method. The regression line was required to be in the same direction as the original association. Quantitative RT-PCR of the control probe sets showing no association with the SNP were also required to be nonsignificant at this threshold. Effect of unannotated SNPs on the analysis. We have previously shown that SNPs located within probes may affect their hybridization to target DNA16, and have therefore conservatively masked out all probes containing SNPs to circumvent this problem. However, probes containing unannotated SNPs are not accounted for; therefore, we wanted to assess the effect of these unknown SNPs on our analysis. We selected 83 genes, each of which contained only a single significant probe set. Many (63) of these probe sets are supported by a single independent, nonoverlapping probe, and such probe sets are the most susceptible to the effect of SNPs, because every probe could potentially be affected by a single SNP. We sequenced the probe sets from the cell lines of six individuals, three from each of the two homozygous genotypes of the associated SNP. We observed that the sequences for 56 probe sets (67.5%) were identical in all samples tested, suggesting that these are more likely to be true events and not an artifact of one or more SNPs located in the individual probes representing the probe set. In the remaining 27 probe sets (32.5%), we identified previously unknown SNPs or indels overlapping one or more of the probes of the probe set, and in most cases, these polymorphisms segregated with one of the two homozygous sample groups, most likely giving rise to the apparent false-positive hit. We excluded these 27 probe sets from our candidate list presented in the manuscript. All of the remaining candidates are supported by two or more independent probes, and are much less susceptible to the effect of unknown SNPs. Only 2 out of the 32 candidates from the final dataset selected for validation (6%) contained previously unidentified SNPs and hence failed validation, showing that the effect of SNPs on the final results presented here is small. URLs. Results from regression analyses at the probe set and meta–probe set levels, including gene-level plots of expression changes, and other relevant information can be found at the GRiD (Genetic Regulators in Disease) website (http://www.regulatorygenomics.org). For the probe logarithmic-intensity error model, see http://www.affymetrix.com/support/technical/technotes/ plier_technote.pdf. Accession codes. US National Center for Biotechnology Information, Gene Expression Omnibus: The data discussed in this publication are accessible through the GEO Series accession number GSE9372. Note: Supplementary information is available on the Nature Genetics website. ACKNOWLEDGMENTS The authors would like to thank D. Serre, T. Pastinen, E. Harmsen and H. Zuzan for helpful discussions and D. Sinnett for technical assistance. This work is supported by Genome Canada, Genome Que´bec and the Canadian Institutes of Health Research (CIHR). 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