循环阵列测序-滚环扩增建库 cyclic-array sequencing a In vitro mate-paired library construction universal 滚环复制 sequences 测序文库构建 1 kb genomic 拷贝扩增 DNA fragment Universal linker paired genomic tags Mme酶切为点顺序 NNASSVESNNNNNNNNNNNNNNNNNNNNNN Mmel digestion half mate-paired genomic sequences i: Sheared, size-selected genomic fragments (yellow)are circularized with a linker(red ) bearing Mme I recognition sites. Subsequent steps, which include a rolling circle amplification, yield the 134-to 136-bp mate-paired library molecules循环阵列测序--滚环扩增建库 cyclic-array sequencing 测 序 文 库 构 建 滚环复制 拷贝扩增