Polymerase colonv. or polony technologies 循环阵列测序PcR建库 I perform multiplex amplification whil maintaining spatial clustering of identical amplicons In emulsion PCr(ePCr),a Emulsion PCR waterin-oil emulsion permits millions of noninteracting amplifications within a millHiliter-scale volume. Amplification products of individual compartments are captured via inclusion of 1-mm paramagnetic beads bearing one of the PCR primers. Any single bead bears thousands of single-stranded copies of the same PCr product, whereas different beads bear the products of different compartmentalized PCr reactions. The beads generated by ePcr have highly desirable characteristics 乳液PR:每一粒乳液含一个磁珠, high signal density, geometric 含一个待扩增的DNA片段 uniformity, strong feature separation, 注: ePCR yields clonal template amplification on and a size that is small but still 1-mm beads resolvable by inexpensive optics.循环阵列测序-PCR建库 Polymerase colony, or polony,technologies perform multiplex amplification while maintaining spatial clustering of identical amplicons. In emulsion PCR (ePCR), a waterin-oil emulsion permits millions of noninteracting amplifications within a milliliter-scale volume . Amplification products of individual compartments are captured via inclusion of 1-mm paramagnetic beads bearing one of the PCR primers . Any single bead bears thousands of single-stranded copies of the same PCR product, whereas different beads bear the products of different compartmentalized PCR reactions . The beads generated by ePCR have highly desirable characteristics: high signal density, geometric uniformity, strong feature separation, and a size that is small but still resolvable by inexpensive optics