A double-reciprocal plot of enzyme kinetics 双倒数曲线 The determination of KM and Vmax values required algebraic manipulation of the basic Michaelis-Menten equation. 1/Vo Slope Km/Vmax 1 KM Intercept =-1/KM Intercept =1/Vmax 0 1/[s] Figure 8.13 A double-reciprocal of Lineweaver-Burk plotFigure 8.13 A double-reciprocal of Lineweaver-Burk plot The determination of KM and Vmax values required algebraic manipulation of the basic Michaelis- Menten equation. A double-reciprocal plot of enzyme kinetics 双倒数曲线