KM and Vmax Values Determination 1.Curve-fitting programs on a computer:The Michaelis constant,KM,and the maximal rate,Vmax,can be readily derived from rates of catalysis measured at a variety of substrate concentrations if an enzyme operates according to the simple scheme given in equation 27.The derivation of KM and Vmax is most commonly achieved with the use of curve-fitting programs on a computer. max s Vo Vmax [S]KM Vmax/2 KM Substrate concentration [S]-KM and Vmax Values Determination 1. Curve-fitting programs on a computer: The Michaelis constant, KM, and the maximal rate, Vmax, can be readily derived from rates of catalysis measured at a variety of substrate concentrations if an enzyme operates according to the simple scheme given in equation 27. The derivation of KM and Vmax is most commonly achieved with the use of curve-fitting programs on a computer