11.2 Sequencing and Annotating Genomes Sequencing:determining the precise order of nucleotides in a DNA or RNA molecule @Sanger dideoxy method桑格双脱氧测序方法 Invented by Nobel Prize winner Fred Sanger Dideoxy analogs of dNTPs used in conjunction with dNTPs Analog prevents further extension of DNA chain Bases are labeled with radioactivity Gel electrophoresis is then performed on products Chen Feng,Lecture of MicrobiologyChen Feng, Lecture of Microbiology 11.2 Sequencing and Annotating Genomes Sequencing: determining the precise order of nucleotides in a DNA or RNA molecule Sanger dideoxy method 桑格双脱氧测序方法 • Invented by Nobel Prize winner Fred Sanger • Dideoxy analogs of dNTPs used in conjunction with dNTPs • Analog prevents further extension of DNA chain • Bases are labeled with radioactivity • Gel electrophoresis is then performed on products