Dideoxynucleotides and Sanger sequencing双脱氧核苷三磷酸与桑格测序法 5 CH2 Base O=P-O-CH2 Base 0 3 Principle: OH H Missing OH- A normal deoxynucleotide has Normal deoxynucleotide Dideoxy analog (a) a hydroxyl group on the 3' carbon and a DNA chain Direction of dideoxynucleotide does not. O-H chain growth 5 0=P-0-CH20 Base Chain termination will occur 0 after incorporation of a dideoxynucleotide. P-O-CH2 Base No free 3'-OH,replication will stop at this point Have been discussed (b) in Lecture 7 2012 Pearson Educaton,ineChen Feng, Lecture of Microbiology Dideoxynucleotides and Sanger sequencing双脱氧核苷三磷酸与桑格测序法 Principle: • A normal deoxynucleotide has a hydroxyl group on the 3’ carbon and a dideoxynucleotide does not. • Chain termination will occur after incorporation of a dideoxynucleotide. Have been discussed in Lecture 7