In the manual sequencing method,a single strand of DNA strand to be sequenced the DNA to be sequenced is 3'CGACTCGATTC5 5'GCTG3 mixed with a primer,DNA Radioactive Add DNA polymerase,mixture DNA primer of all four deoxyribonucleotide polymerase I,four dNTP triphosphates;separate into four reaction tubes (one of which is -A small amount of only one dideoxynucleotide triphosphate(ddGTP,ddATP,ddTTP,or ddCTP) radiolabeled),and a small added to each tube and reaction allowed to proceed Reaction products amount of one of the ddGTP ddATP ddTTP ddCTP dideoxynucleotides. A-G(2) -A(1) AGC-T(4)AG-C(3) AGCTAA-G(7)AGCT-A(5) AGCTA-A(6) A a 2012 Poarson,ine Note that four different reactions must be run,one with each dideoxynucleotide.In the manual sequencing method, a single strand of the DNA to be sequenced is mixed with a primer, DNA polymerase I, four dNTP (one of which is radiolabeled), and a small amount of one of the dideoxynucleotides. Note that four different reactions must be run, one with each dideoxynucleotide