第40卷第2期 张茜冯振卿.新型冠状病毒肺炎的抗体应对策略[J] 2020年2月 南京医科大学学报(自然科学版),2020,40(2):155-159 159 mation[j].biorxiV,2020,doi:https://doi.org/10.1101/ has broad neutralizing potency against H5N1 influenza A 2020.02.11944462 viruses of different clades [j]. Antiviral Res, 2013, 90 [22] ZHOU P, YANG X, WANG X, et al. Discovery of a novel oronavirus associated with the recent pneumonia out- [24] ZHANG X, ZHANG C, LIU Y, et al. Construction of scFv and its potential bat origin [J][J]. hage display library with hapten-specific repertories and BbioRxiv,2020,doi:https://doi.org/10.1101/2020.01.22. characterizationof anti-ivermectin fragment isolated from 914952 the library [J]. Eur Food Res Technol, 2010, 231(3) [23]ZHANG X, QI X, ZHANG Q, et al. Human 4F5 423-430 chain Fy antibody recognizing a conserved HAl 收稿日期]2020-02-18 (上接第154页) ry syndrome coronavirus in nonhuman primates [J].Sc [20]WRAPPD, WANGN, CORBETTK S, et al. 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Vaccine,2008,26(50):6338-6343 [32 LimPL, KURUPA, GOPALAKRISHNAG, et al.Laboral [26 MUTHUMANIK, FALZARANOD, REUSCHELEL, et al acquired severe acute respiratory syndrome [J]. N Engl J A synthetic consensus anti-spike protein DNA vaccine in- Med,2004,350(17):1740-1745 uces protective immunity against Middle East respirato- [收稿日期]200-02-20mation［J］. BioRxiv，2020，doi：https：//doi.org/10.1101/ 2020.02.11.944462 ［22］ ZHOU P，YANG X，WANG X，et al. Discovery of a novel coronavirus associated with the recent pneumonia out⁃ break in humans and its potential bat origin［J］［J］. BBioRxiv，2020，doi：https：//doi.org/10.1101/2020.01.22. 914952 ［23］ ZHANG X，QI X，ZHANG Q，et al. Human 4F5 single ⁃ chain Fv antibody recognizing a conserved HA1 epitope has broad neutralizing potency against H5N1 influenza A viruses of different clades［J］. Antiviral Res，2013，99 （2）：91-99 ［24］ ZHANG X，ZHANG C，LIU Y，et al. Construction of scFv phage display library with hapten⁃specific repertories and characterization of anti⁃ivermectin fragment isolated from the library［J］. Eur Food Res Technol，2010，231（3）： 423-430 ［收稿日期］ 2020-02-18 ［20］WRAPPD，WANGN，CORBETTK S，et al. Cryo⁃EM struc⁃ ture of the 2019⁃nCoV spike in the prefusion conformation ［J］. Science，2020，doi：10.1126/science.abb2507 ［21］ENjUANESL，DEDIEGOML，ALVAREZE，et al. Vaccines to prevent severe acute respiratory syndrome coronavirus⁃ induced disease［J］. Virus Res，2008，133（1）：45-62 ［22］MINAKSHIR，PADHANK，RANIM，et al. The SARS coro⁃ navirus 3a protein causes endoplasmic reticulum stress and induces ligand ⁃ independent downregulation of the type 1 interferon receptor［J］. PLoS One，2009，4（12）： e8342 ［23］LUR，ZHAO X，LIJ，et al. Genomic characterisation and epidemiology of 2019 novel coronavirus：implications for virus origins and receptor binding.［J］. Lancet，2020，doi： 10.1016/S0140⁃6736（20）30251-8 ［24］ YANGZY，KONGWP，HUANGY，et al. A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice［J］. Nature，2004，428（6982）：561-564 ［25］MARTINJE，LOUDERMK，HOLMAN LA，et al. A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a phase I clinical trial［J］. Vaccine，2008，26（50）：6338-6343 ［26］ MUTHUMANIK，FALZARANOD，REUSCHELEL，et al. A synthetic consensus anti⁃spike protein DNA vaccine in⁃ duces protective immunity against Middle East respirato⁃ ry syndrome coronavirus in nonhuman primates［J］. Sci Transl Med，2015，7（301）：301ra132 ［27］ MODjARRADK，ROBERTSCC，MILLSKT，et al. Safety and immunogenicity of an anti ⁃ Middle East respiratory syndrome coronavirus DNA vaccine：a phase 1，open ⁃la⁃ bel，single ⁃ arm，dose ⁃ escalation trial［J］. Lancet Infect Dis，2019，19（9）：1013-1022 ［28］ FYNANEF，LUS，ROBINSONHL，et al. One group's his⁃ torical reflections on dna vaccine development［J］. Hum Gene Ther，2018，29（9）：966⁃970 ［29］ YIPMS，LEUNGNH，CHEUNGCY，et al. Antibody⁃depen⁃ dent infection of human macrophages by severe acute re⁃ spiratory syndrome coronavirus［J］. Virol J，2014，11（1）： 82 ［30］ OLSEN CW，NGICHABE CK，BAINES JD，et al. Mono⁃ clonal antibodies to the spike protein of feline infectious peritonitis virus mediate antibody ⁃ dependent enhance⁃ ment of infection of feline macrophages［J］. JVirol，1992， 66（2）：956-965 ［31］ HOLMESKV. SARS coronavirus：a new challenge for pre⁃ vention and therapy［J］. J Clin Invest，2003，111（11）： 1605-1609 ［32］ LimPL，KURUPA，GOPALAKRISHNAG，et al. Laboratory ⁃acquired severe acute respiratory syndrome［J］. N Engl J Med，2004，350（17）：1740-1745 ［收稿日期］ 2020-02-20 （上接第154页） 􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂􀤂 第40卷第2期 2020年2月 张 茜，冯振卿. 新型冠状病毒肺炎的抗体应对策略［J］. 南京医科大学学报（自然科学版），2020，40（2）：155-159 ·159·