刘玉婷,等.肠道菌群的检测方法及研究进展 162445 钟燕,黄承钰应用荧光原位杂交技术分析乳糖不耐■同行评价 18 Di Giovanni GD, Watrud LS, Seidler R, widmer 受者结肠菌群.营养学报2003;25:415-417 本文综述了肠道 F Fingerprinting of mixed bacterial strain Dobrindt U, Agerer F, Michaelis K, Janka菌群检测方法 and BIOLOG gram-negative(GN) substrate A, Buchrieser c, Samuelson m, Svanborg C,能较全面概括目 communities by enterobacterial repetitive Gottschalk G, Karch h, Hacker. Analysis of前常用的分子生 intergenic consensus sequence-PCR (ERIC-PCR) genome plasticity in pathogenic and commensal物学技术,为选 Curr Microbiol199938:217-223[PMD:10069857 Escherichia coli isolates by use of DNA array 19鲁海峰,魏桂芳,李仲逵.ERIC-PCR分子杂交技术 Bacteriol2003185183180PMD:1261847提供了指导,因 分析大熊猫肠道菌群结构.中国微生物杂志2005; DO:10.1128/JB.1856.1831-1840.2003] 17:81-84 8 J, Puel JL. From cochlear cell death较强的临床意义 Li RM, Han Y, Wang JH, Wang ZH. [An analysis of the DNA fingerprinting of intestinal Rev med clienr20088:1006-1019[PMD:18782053 nflammatory bowel disease]. Zhonghe DO:102174/138955708785740599 Zizhi2007;46:9698[PMD:17445429 36翟俊辉,郭兆彪16rDNA基因芯片检测临床常见 21 Muyzer G DGGE/TGGE a method for 感染性细菌临床检验杂志2002;20:133-136 identifying genes from natural ecosystems.Cm37陈燕飞.肝硬化患者肠道微生态研究:从结构到功 Opin Microbio! 1999; 2: 317-322 [PMID: 10383868 能杭州浙江大学,2013 DO:10.1016/ 38毛正果,郑浩轩,王新颖.基因芯片检测常见肠道 22 Meroth CB, Walter Hertel C, Brandt MJ, 致病菌感染的研究与评价.胃肠病学和肝病学杂志 Hammes WP. Monitoring the bacterial population 08;17: dynamics in sourdough fermentation processes by39王军,逢建涛,刘玉瑜.基囚芯片检测7种常见腹泻 using PCR-denaturing gradient gel electrophoresis. 病致病菌方法研究.中国人兽共患病学报2012;28 pPI Environ Microbiol 2003; 69: 475-482 [PMID: 12514030 DOI: 10.1128/AEM.69.1.475-4822003] 40 Liu Y, Bowen NJ, Matyunina L, McDonald J, 23刘霞.应用 PCR-DGGE和 rep-PCR技术对大鼠及成 Prausnitz MR. Gene transfection enhanced 人肝硬化肝移植后肠道菌群多样性的研究.杭州浙 by ultrasound exposure combined with drug 江大学,2011 treatment guided by gene chip analysis. Int J 24 Dar SA, Kuenen JG, Muyzer G Nested PCR Hyperthermia2012;28:349-361IPMD:22621736 denaturing gradient gel electrophoresis approach to O:103109/026567362012669513 determine the diversity of sulfate-reducing bacteria 41 Osterberg FW, Rizzi G, Donolato M, Bejhed RS elex microbial communities Environ Mezger A, Stromberg M, Nilsson M, Stromme Microbiol2005;71:2325230PMD:15870318 M, Svedlindh P, Hansen M. On-chip detection 帅,王晨阳,范朝刚.变形梯度凝胶电泳研究脓毒 of rolling circle amplified DNA molecules from 症大鼠肠道菌群的变化肠外与肠内营养2014;21: Bacillus globigii spores and Vibrio cholerae 236-240 Small2014;10.2877-2882[PMID:24616417DOL 始6宫曼丽,任南琪邢德峰.DGGE/TGGE技术及其在 1002/smll201303325 微生物分子生态学中的应用微生物学报200444:42 Salonen a, Nikkila j, Jalanka- Tuovinen I 845-848 Immonen O, Rajilic-Stojanovic M, Kekkonen RA, 27 Wei G, Lu H, Zhou Z, Xie H, Wang A, Nelson K, alva A, de Vos WM. Comparative analysis of Zhao L. The microbial community in the feces fecal DNA extraction methods with phylogenetic of the giant panda(Ailuropoda melanoleuca)as microarray: effective recovery of bacterial and determined by PCR-TGGE Profiling and clone archaeal DNA using mechanical cell lysis. J library analysis. Microb Ecol 2007: 54: 194-202 Microbiol Methods 2010; 81: 127-134 [PMID PMD17394039DO110.1007/s00248-007-92252] 0171997DO:10.1016/ g. mimet201002007 28庞小燕,张宝让,魏桂芳.应用TGGE技术分析人肠43 Eckburg PB, Bik em, Bernstein CN, Purdom e 道中双歧杆菌的组成微生物学报2005;:45:738-74 ethlefsen L, Sargent M, Gill SR, Nelson Ke, 29熊婧,张思璐,魏霜.属特异性 T-RFLP技术在双歧 Relman DA. Diversity of the human intestinal 杆菌群落分析中的应用.微生物学通报2014;41 microbial flora. Science 2005: 308: 1635-1638 2538-2546 PMID:15831718DO10.1126/ scIence.1110591] 30 Hakansson A, Tormo-Badia N, Baridi A, Xu J, Manichanh C, Rigottier-Gois L, Bonnaud E, Molin G, Hagslatt ML, Karlsson C, Jeppsson B, Gloux K, Pelletier E, Frangeul L, Nalin R, Jarrin Cilio CM, Ahrne S Immunological alteration and C, Chardon P, Marteau P, Roca J, Dore J Reduced changes of gut microbiota after dextran sulfate diversity of faecal microbiota in Crohns disease dium(DsS) revealed by a metagenomic approach. Gut 2006 led2015;15:107-120[PMID:2441342DoI: 55:205-211[PMID:16188921DOI:10.1136/ 10.1007/s10238-013-02705 gut2005.0738171 31 Wang M, Ahrne S, Antonsson M, Molin G. 45 Qin J, Li R, Raes J, Arumugam M, Burgdorf Ks, T-RFLP combined with principal component Manichanh C, Nielsen T, Pons N, Levene F, analysis and 16s rrNa gene sequencing Yamada T, Mende Dr, Li J, Xu J, LiS, Li D, Cao J, an effective strategy for comparison of fecal Wang B, Liang H, Zheng H, Xie Y, Tap ), Lepage microbiota in infants of different ages. J Microbiol P, Bertalan M, Batto JM, Hansen T, Le Paslier D, Methods2004;5953-69[PMID:15325753DOI: Linneberg A, Nielsen HB, Pelletier E, Renault P, Sicheritz-Ponten T, Turner K, Zhu H, Yu C, Li s, 32柳欣源,申剑,王婷婷.T-RFLP技术在人肠道柔嫩 Jian M, Zhou Y, Li Y, Zhang X, Lis, Qin N, Yang H, 梭菌类群的组成分析中的应用.中国微生态学杂志 Wang J, Brunak S, Dore J, Guarner F, Kristianse 200921:696700 K, Pedersen O, Parkhill J, Weissenbach J, Bork +5Aishideng 3147 2016-07-18 Volume 24 I Issue 20 I刘玉婷, 等. 肠道菌群的检测方法及研究进展 WCJD|www.wjgnet.com 3147 2016-07-18|Volume 24|Issue 20| ■同行评价 本文综述了肠道 菌 群 检 测 方 法 , 能较全面概括目 前常用的分子生 物学技术, 为选 择何种检测方法 提供了指导, 因 此, 本综述具有 较强的临床意义. 1624445] 18 Di Giovanni GD, Watrud LS, Seidler RJ, Widmer F. Fingerprinting of mixed bacterial strains and BIOLOG gram-negative (GN) substrate communities by enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR). Curr Microbiol 1999; 38: 217-223 [PMID: 10069857] 19 鲁海峰, 魏桂芳, 李仲逵. ERIC-PCR分子杂交技术 分析大熊猫肠道菌群结构.中国微生物杂志 2005; 17: 81-84 20 Li RM, Han Y, Wang JH, Wang ZH. [An analysis of the DNA fingerprinting of intestinal flora in inflammatory bowel disease]. Zhonghua Neike Zazhi 2007; 46: 96-98 [PMID: 17445429] 21 M u y z e r G . D G G E / T G G E a m e t h o d f o r identifying genes from natural ecosystems. Curr Opin Microbiol 1999; 2: 317-322 [PMID: 10383868 DOI: 10.1016/S1369-5274(99)80055-1] 22 Meroth CB, Walter J, Hertel C, Brandt MJ, Hammes WP. Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol 2003; 69: 475-482 [PMID: 12514030 DOI: 10.1128/AEM.69.1.475-482.2003] 23 刘霞. 应用PCR-DGGE和rep-PCR技术对大鼠及成 人肝硬化肝移植后肠道菌群多样性的研究. 杭州: 浙 江大学, 2011 24 Dar SA, Kuenen JG, Muyzer G. Nested PCR￾denaturing gradient gel electrophoresis approach to determine the diversity of sulfate-reducing bacteria in complex microbial communities. Appl Environ Microbiol 2005; 71: 2325-2330 [PMID: 15870318] 25 周帅, 王晨阳, 范朝刚. 变形梯度凝胶电泳研究脓毒 症大鼠肠道菌群的变化.肠外与肠内营养 2014; 21: 236-240 26 宫曼丽, 任南琪, 邢德峰. DGGE/TGGE技术及其在 微生物分子生态学中的应用. 微生物学报 2004; 44: 845-848 27 Wei G, Lu H, Zhou Z, Xie H, Wang A, Nelson K, Zhao L. The microbial community in the feces of the giant panda (Ailuropoda melanoleuca) as determined by PCR-TGGE profiling and clone library analysis. Microb Ecol 2007; 54: 194-202 [PMID: 17394039 DOI: 10.1007/s00248-007-9225-2] 28 庞小燕, 张宝让, 魏桂芳. 应用TGGE技术分析人肠 道中双歧杆菌的组成. 微生物学报 2005; 45: 738-743 29 熊婧, 张思璐, 魏霜. 属特异性T-RFLP 技术在双歧 杆菌群落分析中的应用. 微生物学通报 2014; 41: 2538-2546 30 Håkansson Å, Tormo-Badia N, Baridi A, Xu J, Molin G, Hagslätt ML, Karlsson C, Jeppsson B, Cilio CM, Ahrné S. Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice. Clin Exp Med 2015; 15: 107-120 [PMID: 24414342 DOI: 10.1007/s10238-013-0270-5] 31 Wang M, Ahrné S, Antonsson M, Molin G. T-RFLP combined with principal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages. J Microbiol Methods 2004; 59: 53-69 [PMID: 15325753 DOI: 10.1016/j.mimet.2004.06.002] 32 柳欣源, 申剑, 王婷婷. T-RFLP技术在人肠道柔嫩 梭菌类群的组成分析中的应用. 中国微生态学杂志 2009; 21: 696-700 33 钟燕, 黄承钰. 应用荧光原位杂交技术分析乳糖不耐 受者结肠菌群. 营养学报 2003; 25: 415-417 34 Dobrindt U, Agerer F, Michaelis K, Janka A, Buchrieser C, Samuelson M, Svanborg C, Gottschalk G, Karch H, Hacker J. Analysis of genome plasticity in pathogenic and commensal Escherichia coli isolates by use of DNA arrays. J Bacteriol 2003; 185: 1831-1840 [PMID: 12618447 DOI: 10.1128/JB.185.6.1831-1840.2003] 35 Wang J, Puel JL. From cochlear cell death pathways to new pharmacological therapies. Mini Rev Med Chem 2008; 8: 1006-1019 [PMID: 18782053 DOI: 10.2174/138955708785740599] 36 翟俊辉, 郭兆彪. 16SrDNA基因芯片检测临床常见 感染性细菌. 临床检验杂志 2002; 20: 133-136 37 陈燕飞. 肝硬化患者肠道微生态研究: 从结构到功 能. 杭州: 浙江大学, 2013 38 毛正果, 郑浩轩, 王新颖. 基因芯片检测常见肠道 致病菌感染的研究与评价. 胃肠病学和肝病学杂志 2008; 17: 809-812 39 王军, 逢建涛, 刘玉瑜. 基囚芯片检测7种常见腹泻 病致病菌方法研究. 中国人兽共患病学报 2012; 28: 938-941 40 Liu Y, Bowen NJ, Matyunina L, McDonald J, Prausnitz MR. Gene transfection enhanced by ultrasound exposure combined with drug treatment guided by gene chip analysis. Int J Hyperthermia 2012; 28: 349-361 [PMID: 22621736 DOI: 10.3109/02656736.2012.669513] 41 Østerberg FW, Rizzi G, Donolato M, Bejhed RS, Mezger A, Strömberg M, Nilsson M, Strømme M, Svedlindh P, Hansen MF. On-chip detection of rolling circle amplified DNA molecules from Bacillus globigii spores and Vibrio cholerae. Small 2014; 10: 2877-2882 [PMID: 24616417 DOI: 10.1002/smll.201303325] 42 Salonen A, Nikkilä J, Jalanka-Tuovinen J, Immonen O, Rajilić-Stojanović M, Kekkonen RA, Palva A, de Vos WM. Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis. J Microbiol Methods 2010; 81: 127-134 [PMID: 20171997 DOI: 10.1016/j.mimet.2010.02.007] 43 Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA. Diversity of the human intestinal microbial flora. Science 2005; 308: 1635-1638 [PMID: 15831718 DOI: 10.1126/science.1110591] 44 Manichanh C, Rigottier-Gois L, Bonnaud E, Gloux K, Pelletier E, Frangeul L, Nalin R, Jarrin C, Chardon P, Marteau P, Roca J, Dore J. Reduced diversity of faecal microbiota in Crohn's disease revealed by a metagenomic approach. Gut 2006; 55: 205-211 [PMID: 16188921 DOI: 10.1136/ gut.2005.073817] 45 Qin J, Li R, Raes J, Arumugam M, Burgdorf KS, Manichanh C, Nielsen T, Pons N, Levenez F, Yamada T, Mende DR, Li J, Xu J, Li S, Li D, Cao J, Wang B, Liang H, Zheng H, Xie Y, Tap J, Lepage P, Bertalan M, Batto JM, Hansen T, Le Paslier D, Linneberg A, Nielsen HB, Pelletier E, Renault P, Sicheritz-Ponten T, Turner K, Zhu H, Yu C, Li S, Jian M, Zhou Y, Li Y, Zhang X, Li S, Qin N, Yang H, Wang J, Brunak S, Doré J, Guarner F, Kristiansen K, Pedersen O, Parkhill J, Weissenbach J, Bork