吴凌峰,等成体神经于细胞与微环境 Wu Ling-feng*, Studying for RESULTS AND CONCLUSION: By screening title and abstract contents, papers with original, reliable and mprehensive analysis were selected, and repetitive research and poor quality articles were excluded. There physician, Department of ere 379 English papers and 131 Chinese papers. Finally, only 64 papers were included in result analysis. Adult Neurology, People's Hospital of neural stem cells are closely related to the major cellular components of the microenviroment(vessels anxi Province, Nanchang endothelial cells, astrocytes and ependymal cells)and the extracellular matrix in which they are embedded playing an important role in the regulation of adult neural stem cell survival, proliferation, differentiation. At the wusky2000@126.com same time, the differentiation of adult neural stem cells is regulated by genes. It will produce a new direction of nerve tissue repair between the study of adult neural stem cells and the microenvironment. orresponding author: Wu ey Words: stem cells; stem cell academic discussion; neural stem cells, adult neural stem cells; central nervous Chie physician, system; microenvironment; regulation nerve tissue repair; National Natural Science Foundation of China ople's Hospital of angei Province, Nanchang Wu LF, Wu XM. Adult neural stem cells and the microenvironment. Zhongguo Zuzhi Gongcheng Yanjiu 2013 330006, 17(19):35383545 China m79@163com 0引言 China. No. 81160148 Received: 2013-02-21 神经组织的丧失是由不同病因所致,包括创伤、脑血管病损伤和神经系统变性病等, 会出现累及多个神经系统的临床表现,如肢体运动障碍、感觉障碍、失语及认知功能下 降等。大量临床试验表明,干细胞移植对于改善动物的临床预后具有肯定的作用,如将 骨髓间充质干细胞移植于建模后30min,3h,3d的脑梗死大鼠模型,结果均发现受损 神经功能均有明显好转-,同时,人们也发现中枢神经系统可通过自身内源性干细胞来 修复冏。 Baram等冏认为成体神经发生是在血管源性的微环境中进行的,并影响神经干 细胞的增殖和分化。文章就成体神经干细胞与微环境的研究近况作一综述。 1资料和方法 11资料来源由第一作者检索 PubMed(1989至2012年)数据库与中国期刊全文数据库 CNK:2001至2012年),检索词分别为“神经干细胞;微环境;调控”和“ neural stem cells; microenviroment; regulation”。文献检索语种限制为英文和中文 12入选标准 ①具有原创性,论点论据可靠的成体神经干细胞与微环境文章。②文献主 题内容与成体神经干细胞、微环境联系较紧密的文章。 排除标准:①Meta分析。②原始文献不严谨、统计学方法不恰当或重复性研究 1.3质量评估对每一篇符合纳入标准的文献进行以下几个方面的评价:①相关研究的 新颖性、独特性。②与神经干细胞、微环境的相关性。 14数据的提取共检索到379篇英文文章,131篇中文文章,按纳入及排除标准筛选后, 共纳入64篇文章,主要是成体神经干细胞及微环境的生物特性及成体神经干细胞维持和 分化机制的研究,包括綜述和基础、临床实验研究。 2结果 2.1成体神经干细胞的定义神经干细胞是存在于成体脑组织中的一种干细胞,是具有分 裂潜能和自我更新能力的母细胞,可以通过不对等的分裂方式产生神经组织的各类细 /SSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 3539 o1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp:/www.cnki.net吴凌峰，等. 成体神经干细胞与微环境 ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 3539 www.CRTER.org Wu Ling-feng☆, Studying for doctorate, Associate chief physician, Department of Neurology, People’s Hospital of Jiangxi Province, Nanchang 330006, Jiangxi Province, China wusky2000@126.com Corresponding author: Wu Xiao-mu, M.D., Chief physician, Professor, Department of Neurology, People’s Hospital of Jiangxi Province, Nanchang 330006, Jiangxi Province, China wuxm79@163.com Supported by: the National Natural Science Foundation of China, No. 81160148* Received: 2013-02-21 Accepted: 2013-03-27 RESULTS AND CONCLUSION: By screening title and abstract contents, papers with original, reliable and comprehensive analysis were selected, and repetitive research and poor quality articles were excluded. There were 379 English papers and 131 Chinese papers. Finally, only 64 papers were included in result analysis. Adult neural stem cells are closely related to the major cellular components of the microenviroment (vessels, endothelial cells, astrocytes and ependymal cells) and the extracellular matrix in which they are embedded, playing an important role in the regulation of adult neural stem cell survival, proliferation, differentiation. At the same time, the differentiation of adult neural stem cells is regulated by genes. It will produce a new direction of nerve tissue repair between the study of adult neural stem cells and the microenvironment. Key Words: stem cells; stem cell academic discussion; neural stem cells; adult neural stem cells; central nervous system; microenvironment; regulation; nerve tissue repair; National Natural Science Foundation of China Wu LF, Wu XM. Adult neural stem cells and the microenvironment. Zhongguo Zuzhi Gongcheng Yanjiu. 2013; 17(19):3538-3545. 0 引言 神经组织的丧失是由不同病因所致，包括创伤、脑血管病损伤和神经系统变性病等， 会出现累及多个神经系统的临床表现，如肢体运动障碍、感觉障碍、失语及认知功能下 降等。大量临床试验表明，干细胞移植对于改善动物的临床预后具有肯定的作用，如将 骨髓间充质干细胞移植于建模后30 min，3 h，3 d的脑梗死大鼠模型，结果均发现受损 神经功能均有明显好转[1-4]，同时，人们也发现中枢神经系统可通过自身内源性干细胞来 修复[5]。Barami等[6]认为成体神经发生是在血管源性的微环境中进行的，并影响神经干 细胞的增殖和分化。文章就成体神经干细胞与微环境的研究近况作一综述。 1 资料和方法 1.1 资料来源 由第一作者检索PubMed(1989至2012年)数据库与中国期刊全文数据库 (CNKI：2001至2012年),检索词分别为“神经干细胞；微环境；调控”和“neural stem cells； microenviroment；regulation”。文献检索语种限制为英文和中文。 1.2 入选标准 纳入标准：①具有原创性，论点论据可靠的成体神经干细胞与微环境文章。②文献主 题内容与成体神经干细胞、微环境联系较紧密的文章。 排除标准：①Meta分析。②原始文献不严谨、统计学方法不恰当或重复性研究。 1.3 质量评估 对每一篇符合纳入标准的文献进行以下几个方面的评价：①相关研究的 新颖性、独特性。②与神经干细胞、微环境的相关性。 1.4 数据的提取 共检索到379篇英文文章，131篇中文文章，按纳入及排除标准筛选后， 共纳入64篇文章，主要是成体神经干细胞及微环境的生物特性及成体神经干细胞维持和 分化机制的研究，包括综述和基础、临床实验研究。 2 结果 2.1 成体神经干细胞的定义 神经干细胞是存在于成体脑组织中的一种干细胞，是具有分 裂潜能和自我更新能力的母细胞，可以通过不对等的分裂方式产生神经组织的各类细