《酶制剂与酶工程(英)》教学大纲 (Enzyme Engineering) 、基本信息 课程代码:1040822 学分: 总课时:32 课程性质:专业选修课 适用专业:生物工程本科 先修课程:微生物学,生物化学 本课程的教学目的和任务 The Goal of this course: By studying this course, students can under reparation and purification, and immobilization of enzymes. Also, knowing of applications and reactors of enzyme is another important task of students. Finally, students should master something in enzyme catalysis in organic media and some common, useful industrial enzymes 三、教学方法与手段 本课程采用多媒体教学手段进行课堂讲授,辅以读写议等方法。 四、教学内容与要求 Chapter 1: Fundamentals of enzyme kinetics Students should master: the mechanism of enzyme action and the simple kinetics of enzyme action. Students should understand enzyme inhibition and effect of pH and temperature on Key point of this chapter: the simple kinetics of enzyme action Difficult point in this chapter: Determination of Vmax and K, Chapter 2 Enzyme preparation and purification Students should master the main useful methods of preparation and purification of enzymes Students should understand screening methods of enzymes and measurements of enzyme activity Key point of this chapter: the main useful methods of preparation and purification of enzymes, such as cell breakage, use of lytic methods, filtration, and chromatography Difficult point in this chapter: affinity chromatography Chapter 3 Enzyme Immobilization Students should master: the methods of enzyme immobilization and kinetics of immobilized enzyme. Students should understand common usefulness of immobilized enzymes http:/spxy.zjgsu.edu.cnhttp://spxy.zjgsu.edu.cn 《酶制剂与酶工程（英）》教学大纲 (Enzyme Engineering) 一、基本信息 课程代码：1040822 学 分：2 总 课 时：32 课程性质：专业选修课 适用专业：生物工程本科 先修课程：微生物学，生物化学 二、本课程的教学目的和任务 The Goal of this course: By studying this course, students can understanding kinetics, preparation and purification, and immobilization of enzymes. Also, knowing of applications and reactors of enzyme is another important task of students. Finally, students should master something in enzyme catalysis in organic media and some common, useful industrial enzymes. 三、教学方法与手段 本课程采用多媒体教学手段进行课堂讲授,辅以读写议等方法。 四、教学内容与要求 Chapter 1: Fundamentals of enzyme kinetics Students should master: the mechanism of enzyme action and the simple kinetics of enzyme action. Students should understand enzyme inhibition and effect of pH and temperature on enzyme catalysis. Key point of this chapter: the simple kinetics of enzyme action. Difficult point in this chapter: Determination of Vmax and Km Chapter 2 Enzyme preparation and purification Students should master: the main useful methods of preparation and purification of enzymes. Students should understand screening methods of enzymes and measurements of enzyme activity. Key point of this chapter: the main useful methods of preparation and purification of enzymes, such as cell breakage, use of lytic methods, filtration, and chromatography. Difficult point in this chapter: affinity chromatography Chapter 3 Enzyme Immobilization Students should master: the methods of enzyme immobilization and kinetics of immobilized enzyme. Students should understand common usefulness of immobilized enzymes