Key point of this chapter: the methods of enzyme immobilization and kinetics of lmn Difficult point in this chapter: kinetics of immobilized enzymes Chapter 4: The large-scale use of enzymes in solution Students should master: the most useful applications of enzymes in food industry, leather and wool industry, and detergents. Students should understand the most useful applications of enzymes in food industry, leather and wool industry, and detergents Key point of this chapter: the useful application of enzymes in food and dairy industry Difficult point in this chapter: medical applications of enzymes Chapter 5 Enzyme reactors Students should master the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors Students should understand continuous flow reactors and continuous flow stirred tank reactors Key point of this chapter: the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors Difficult point in this chapter: continuous flow stirred tank reactors Chapter 6 Some important industrial enzymes Students should master: the most important industrial enzymes such as amylase and protease Students should understand the production of lipase and phytase Key point of this chapter: the production and main applications of amylase and protease Difficult point in this chapter: molecular biology of xylanase and phytase Chapter 7 Enzyme catalysis in organic media Students should master: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organic systems. Students should understand the advantages of enzyme catalysis in organic media and some practical uses of enzyme catalysis in organic media. Key point of this chapter: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organIc systems Difficult point in this chapter: Determination of Vmax and Kmkinetics of enzyme catalysis Chapter 8: Future prospects for enzyme technology Students should master: the future prospects of enzyme engineering. Students should Key point of this chapter: the future prospects of enzyme engineering 五、教学时数分配表 Chapters Titles Reference time units Fundamentals of enzyme and enzyme kinetics Enzy eparation and purification http:/spxy.zjgsu.edu.cnhttp://spxy.zjgsu.edu.cn Key point of this chapter: the methods of enzyme immobilization and kinetics of immobilized enzyme. Difficult point in this chapter: kinetics of immobilized enzymes. Chapter 4: The large-scale use of enzymes in solution Students should master: the most useful applications of enzymes in food industry, leather and wool industry, and detergents. Students should understand the most useful applications of enzymes in food industry, leather and wool industry, and detergents. Key point of this chapter: the useful application of enzymes in food and dairy industry. Difficult point in this chapter: medical applications of enzymes. Chapter 5 Enzyme reactors Students should master: the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors. Students should understand continuous flow reactors and continuous flow stirred tank reactors. Key point of this chapter: the main reactors used in production of enzymes, such as membrane reactor, packed bed reactor, and fluidized bed reactors. Difficult point in this chapter: continuous flow stirred tank reactors. Chapter 6 Some important industrial enzymes Students should master: the most important industrial enzymes such as amylase and protease. Students should understand the production of lipase and phytase. Key point of this chapter: the production and main applications of amylase and protease. Difficult point in this chapter: molecular biology of xylanase and phytase. Chapter 7 Enzyme catalysis in organic media Students should master: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organic systems. Students should understand the advantages of enzyme catalysis in organic media and some practical uses of enzyme catalysis in organic media.. Key point of this chapter: the mechanism of enzyme reaction in biphasic liquid systems and the simple kinetics of enzyme catalysis in aqueous-organic systems. Difficult point in this chapter: Determination of Vmax and Kmkinetics of enzyme catalysis in organic media. Chapter 8: Future prospects for enzyme technology Students should master: the future prospects of enzyme engineering. Students should understand where enzyme engineering will go? Key point of this chapter: the future prospects of enzyme engineering. 五、教学时数分配表 Chapters Titles Reference time units 1 Fundamentals of enzyme and enzyme kinetics 4 2 Enzyme preparation and purification 4