4. What are the molecular mechanisms that keep the dna replication error rate as low as 10-9 in cells? 5. Compare the mechanism and consequence of site-specific recombination and transposition 6. Use two examples to illustrate that bacterial gene regulation can occur at and after the transcription initiation 7. Describe the molecular events occurred at a replication fork? Why the replication fork can move to one direction although the leading strand and lagging strand are antiparallel and both synthesized in the 5-3 direction? PART IV: MAJOR QUESTIONS(12.5 points each) 1: Compare the initiation, elongation and termination of the transcription of a protein-encod ing gene in prokaryote and eukaryote 2: After you learned the molecular biology class, you are very interested in studying function of the eukaryotic Tra-2 protein. Obviously, the first challenge is to obtain a large amount of pure Tra-2 protein. Please describe the procedures that you would like to use in order to get the desired Tra-2 protein4. What are the molecular mechanisms that keep the DNA replication error rate as low as 10-9 in cells? 5. Compare the mechanism and consequence of site-specific recombination and transposition. 6. Use two examples to illustrate that bacterial gene regulation can occur at and after the transcription initiation. 7. Describe the molecular events occurred at a replication fork? Why the replication fork can move to one direction although the leading strand and lagging strand are antiparallel and both synthesized in the 5’-3’ direction? PART IV: MAJOR QUESTIONS (12.5 points each) 1: Compare the initiation, elongation and termination of the transcription of a protein-encoding gene in prokaryote and eukaryote. 2: After you learned the molecular biology class, you are very interested in studying function of the eukaryotic Tra-2 protein. Obviously, the first challenge is to obtain a large amount of pure Tra-2 protein. Please describe the procedures that you would like to use in order to get the desired Tra-2 protein