武汉大学生命科学学院 2004-2005学年第二学期期末考试 《分子生物学》试卷 Final exam of Molecular Biology Course(Spring 2005) 年级专业 学号 姓名成绩 PART I: DESCRIPTiON (2.5 points each) Your answer should describe what each item is and how it functions in the cell. diagrams structure and sequence information could be included in your answer, as necessa 1. Affinity chromotography 2 ORNA 3. Alternative splicing 4. Insulator 5. Riboswitch 6. Wobble concept 7.T 8. Replisome 9. tRNA charging 10. tmRNA PARTI: SINGLE CHOICES (2 points each) 1. Weak chemical interactions are essential in the biological system which of the following statement about weak chemical interaction is NOT TRUE? 1) Van der Waals forces and hydrogen bonds are all weak chemical interactions 2) Weak bonds are constantly made and broken at physiological temperature 3) Hydrophobic interactions stabilize macromolecules 4)Covalent bonds are the important forces for protein-protein interaction 2. Which of the following statements on DNA structure is TRUE? 1)DNA can only form both right-handed helices 2) The major groove of dNa helix contains more chemical information than the minor groove does 3) All dna bind ing proteins recognize the major grooves 4)Linking number is changeable property for a cccDNA 3. RNA can fold up to complex tertiary structure because 1)RNA chain is usually single-stranded and form long-range interactions in add ition to local double helical structure 2)RNA contains ribose and uracil 3)Some rNas are enzymes 4) RNA can form pseudoknot structure 4. Which of the following factors does not contribute to the decreased gene density in
武汉大学生命科学学院 2004－2005 学年第二学期期末考试 《分子生物学》试卷 Final exam of Molecular Biology Course (Spring 2005) 年级______ 专业________ 学号_________ 姓名_______成绩______ PART I: DESCRIPTION (2.5 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information could be included in your answer, as necessary. 1. Affinity chromotography 2. microRNA 3. Alternative splicing 4. Insulator 5. Riboswitch 6. Wobble concept 7. Topoisomerase 8. Replisome 9. tRNA charging 10. tmRNA PART II: SINGLE CHOICES (2 points each) 1. Weak chemical interactions are essential in the biological system. Which of the following statement about weak chemical interaction is NOT TRUE? 1) Van der Waals forces and hydrogen bonds are all weak chemical interactions. 2) Weak bonds are constantly made and broken at physiological temperature. 3) Hydrophobic interactions stabilize macromolecules. 4) Covalent bonds are the important forces for protein-protein interaction. 2. Which of the following statements on DNA structure is TRUE? 1) DNA can only form both right-handed helices. 2) The major groove of DNA helix contains more chemical information than the minor groove does. 3) All DNA binding proteins recognize the major grooves. 4) Linking number is changeable property for a cccDNA. 3. RNA can fold up to complex tertiary structure because 1) RNA chain is usually single-stranded and form long-range interactions in addition to local double helical structure. 2) RNA contains ribose and uracil. 3) Some RNAs are enzymes. 4) RNA can form pseudoknot structure. 4. Which of the following factors does NOT contribute to the decreased gene density in eukaryotes?
1)Increase in 2)Increase in intergenic sequences 3)Increase in the intron sequences 4) Increase in ge wide rep 5)Increase in the telomere sequence and complexity 5. The chromosome element important for chromosome segregation 1)Telomere 2)Centro 4)Replication fork 6. Which of the following persons won the Nobel prize because of his/her contribution in discovery of transposable element? 1)Barbara McClintock 2)Joan Steitz 3)Francis Crick 4)Philip Sharp 7. Which of the following mechanisms is not related to Dna damage repair? 1)B 2) Nucleotide excision repair 3)Translesion repair 4)Mismatch repair 5)Double-stand break repair pathway 9. Which of the following statements for translation is NOT CORRECT? 1) Ribosome contains both the decoding center and peptidyl transferase center 2) The peptidyl transferase activity is resided in the large ribosomal RNA 3) Ribosome can directly interpret the genetic information on the mRNa into the corresponding amino acids sequence 4) Peptide bond formation and EF-G drive translocation of the tRNa and the mrNa 10. Which of the following statements on homologous replication is NOT CORRECT? 1)Holliday model illustrates key steps in homologous recombination 2)Homologous recombination is often initiated by double-stranded breaks in DNA It is known that Spol l generates double-stranded breaks in eukaryote 3) RecBCd helicase/nuclease processes broken DNa molecules and generates a single-stranded end 4)Ruv aB complex and Dmcl specifically promotes branch migration in bacteria and eukaryotes, respectively PART III: SHORT QUESTIONS (CHOOSE FIVE QUESTIONS TO ANSWER) (6 points each) 1. What is the central dogma? 2.C he chemistry and mechanism of splicing of the common pre-mRNA introns and group I and group II introns 3. Briefly describe or illustrate how an extracellular signal can alter intracellular gene expre
1) Increase in gene size 2) Increase in intergenic sequences 3) Increase in the intron sequences 4) Increase in genome-wide repeat sequence 5) Increase in the telomere sequence and complexity 5. The chromosome element important for chromosome segregation is 1) Telomere 2) Centromere 3) Origin of replication 4) Replication fork 6. Which of the following persons won the Nobel prize because of his/her contribution in discovery of transposable element? 1) Barbara McClintock 2) Joan Steitz 3) Francis Crick 4) Philip Sharp 7. Which of the following mechanisms is not related to DNA damage repair? 1) Base excision repair 2) Nucleotide excision repair 3) Translesion repair 4) Mismatch repair 5) Double-stand break repair pathway 9. Which of the following statements for translation is NOT CORRECT? 1) Ribosome contains both the decoding center and peptidyl transferase center 2) The peptidyl transferase activity is resided in the large ribosomal RNA 3) Ribosome can directly interpret the genetic information on the mRNA into the corresponding amino acids sequence 4) Peptide bond formation and EF-G drive translocation of the tRNA and the mRNA 10. Which of the following statements on homologous replication is NOT CORRECT? 1) Holliday model illustrates key steps in homologous recombination 2) Homologous recombination is often initiated by double-stranded breaks in DNA. It is known that Spo11 generates double-stranded breaks in eukaryote. 3) RecBCD helicase/nuclease processes broken DNA molecules and generates a single-stranded end. 4) RuvAB complex and Dmc1 specifically promotes branch migration in bacteria and eukaryotes, respectively. PART III: SHORT QUESTIONS (CHOOSE FIVE QUESTIONS TO ANSWER) (6 points each) 1. What is the central dogma? 2. Compare the chemistry and mechanism of splicing of the common pre-mRNA introns and group I and group II introns. 3. Briefly describe or illustrate how an extracellular signal can alter intracellular gene expression
4. What are the molecular mechanisms that keep the dna replication error rate as low as 10-9 in cells? 5. Compare the mechanism and consequence of site-specific recombination and transposition 6. Use two examples to illustrate that bacterial gene regulation can occur at and after the transcription initiation 7. Describe the molecular events occurred at a replication fork? Why the replication fork can move to one direction although the leading strand and lagging strand are antiparallel and both synthesized in the 5-3 direction? PART IV: MAJOR QUESTIONS(12.5 points each) 1: Compare the initiation, elongation and termination of the transcription of a protein-encod ing gene in prokaryote and eukaryote 2: After you learned the molecular biology class, you are very interested in studying function of the eukaryotic Tra-2 protein. Obviously, the first challenge is to obtain a large amount of pure Tra-2 protein. Please describe the procedures that you would like to use in order to get the desired Tra-2 protein
4. What are the molecular mechanisms that keep the DNA replication error rate as low as 10-9 in cells? 5. Compare the mechanism and consequence of site-specific recombination and transposition. 6. Use two examples to illustrate that bacterial gene regulation can occur at and after the transcription initiation. 7. Describe the molecular events occurred at a replication fork? Why the replication fork can move to one direction although the leading strand and lagging strand are antiparallel and both synthesized in the 5’-3’ direction? PART IV: MAJOR QUESTIONS (12.5 points each) 1: Compare the initiation, elongation and termination of the transcription of a protein-encoding gene in prokaryote and eukaryote. 2: After you learned the molecular biology class, you are very interested in studying function of the eukaryotic Tra-2 protein. Obviously, the first challenge is to obtain a large amount of pure Tra-2 protein. Please describe the procedures that you would like to use in order to get the desired Tra-2 protein
