● Molecular iology course Quiz roles (1)桌上只许有笔,答题纸和英文课本。(2)不许 离开自己的座位。(3)不许偷看,不许讲话(有问题 举手)(4)老师宣布停的时候,必须放下自己的笔。 (5)老师宣布收试卷时,试卷必须快速由每排的最左 边传向右边,每排最右边的同学负责把试卷交给当堂 助教。 如果主讲老师或助教发现任何学生违反以上任何 条规则,第一次违规者,扣除总考试成绩的15% 第二次违规者必须重修这们课。 @劣博字 Wuhan Universit
Quiz Roles •Molecular Biology Course （1）桌上只许有笔，答题纸和英文课本。（2）不许 离开自己的座位。（3）不许偷看，不许讲话 (有问题 举手）（4）老师宣布停的时候，必须放下自己的笔。 （5）老师宣布收试卷时，试卷必须快速由每排的最左 边传向右边，每排最右边的同学负责把试卷交给当堂 助教。 如果主讲老师或助教发现任何学生违反以上任何 一条规则，第一次违规者，扣除总考试成绩的15%， 第二次违规者必须重修这们课
Molecular tology course Quiz 1: Section K-L 50 min 1.(20)Compare the processes of dna replication and transcription how do they differ in terms of the enzymes involved, the fidelity of the reaction and the nature of the molecules roduced by each reaction? What do the processes have in common 2.(25)What are the major trans-acting factors and cis-acting elements involved in E. coli transcription? How they interact with each other to carry out the transcription process? Describe the roles of the each subunit when you get to the e coli rna polymerase
Quiz 1: Section K-L 1. (20)Compare the processes of DNA replication and transcription. How do they differ in terms of the enzymes involved, the fidelity of the reaction, and the nature of the molecules produced by each reaction? What do the processes have in common? 2. (25) What are the major trans-acting factors and cis-acting elements involved in E. coli transcription? How they interact with each other to carry out the transcription process? Describe the roles of the each subunit when you get to the E. coli RNA polymerase. •Molecular Biology Course 50 min
3.(10) Regulation of gene expression in prokaryote usually occurs at the transcription level, and the two major mechanisms are and 4.(15)What are the mechanisms that ensure the structure genes of lac operon are only transcribed at a full speed when lactose is the only carbon source. Detail the molecular interactions and events in each mechanism 5.(15) How the control strategy used by trp operon differs from that of lac operon, why?
3. (10) Regulation of gene expression in prokaryote usually occurs at the transcription ___level, and the two major mechanisms are___ and ___ . 4. (15) What are the mechanisms that ensure the structure genes of lac operon are only transcribed at a full speed when lactose is the only carbon source. Detail the molecular interactions and events in each mechanism. 5. (15) How the control strategy used by trp operon differs from that of lac operon, why?
6.(15)An operon in E. coli is controlled by a repressor that binds at the two operator sites (O, and O2 diagrammed in the figure below In the presence of the appropriate inducer, a transcription rate of 100 is observed, but in the absence of inducer, the transcription rate falls to 5. if either of the two sites is mutated so that the repressor cannot bind then the transcription rate is observed to be 100 Additionally, if base pairs are inserted at the arrow, the level of transcription is found to vary with the size of the insert as shown in the graph below. Briefly explain this data
6. (15) An operon in E. coli is controlled by a repressor that binds at the two operator sites (O1 and O2 ) diagrammed in the figure below. In the presence of the appropriate inducer, a transcription rate of 100 is observed, but in the absence of inducer, the transcription rate falls to 5. If either of the two sites is mutated so that the repressor cannot bind, then the transcription rate is observed to be 100. Additionally, if base pairs are inserted at the arrow, the level of transcription is found to vary with the size of the insert as shown in the graph below. Briefly explain this data
O insertion point 100 5 0 5 0 15 21 size of insert(bp)