不同粘性末端的连接 CTGCAG GGATCC GGATCC GACGTC CCTAGG CCTAGG Pstl BamHI 5 CTGCA 3 5 GATCC G G 3’ ACGTO CCTAG 5 T4-DNA pol 切平 Klenow 补平 G 5 GATCC GGATC G CTAGG CCTAG 5 T4-DNA ligase GGATCG CALCO GGATCG CCTAGC不同粘性末端的连接 不同粘性末端的连接 BamHI BamHI 5' 5' GGATCC CCTAGG GGATCC CCTAGG 5' G CCTAG GATCC G 5' CTGCA 5' G 5' G ACGTC 3' T4-DNA ligase T4-DNA ligase CGATCC GCTAG G 5' 5' GGATC G CCTAG C 5' CTGCAG GACGTC 5' PstI PstI 3' T4-DNA pol T4-DNA pol 切平切平 5' C G 5' G C Klenow Klenow 补平补平 5' GGATC CCTAG 5' GATCC CTAG G CGATCC GCTAG G 5' 5' GGATC G CCTAG C