16.7 Evolutionary Analysis: Analytical Methods Isolate DNA 16S gene Amplify 16S gene by PCR Most scientists today employ Run on agarose gel:check for correct size PCR to amplify directly the Kilo- bases 1 2 3.0- genes encoding 16S rRNA from 2.0- 1.5- genomic DNA and then 1.0- sequence the PCR product by 0.5- Sequence standard dideoxy DNA sequencing Align sequences; generate tree Distinct Ancestral species cell Sequencing Methodology Distinct species of rRNArRNA的测序方法 2012 Pearson Education,IncMost scientists today employ PCR to amplify directly the genes encoding 16S rRNA from genomic DNA and then sequence the PCR product by standard dideoxy DNA sequencing. Sequencing Methodology of rRNA rRNA的测序方法 16.7 Evolutionary Analysis: Analytical Methods