Generating a phylogenetic trees from 16S rRNA sequences Organism Sequence Analysis CGUAGACCUGAC ForA→B,three differences occur CCUAGAGCUGGC out of a total of FLASH twelve;thus=0.25 CCAAGACGUGGC D GCUAGAUGUGCC (a)Sequence alignment and analysis Evolutionary distance Corrected evolutionary distance Ep A B 0.25 0.30 Ep A 0.33 0.44 0.42 0.61 -0.23 0.31- 0.25 0.30 0.08H B Eo B 0.33 0.44 -0.08+ -0.15 Ep 0.33 0.44 0.29 (b)Calculation of evolutionary distance (c)Phylogenetic treeChen Feng, Shanghai Jiao Tong University Generating a phylogenetic trees from 16S rRNA sequences FLASH