TCRy962(OT3)Fc融合蛋白体内抗肿瘤效果研究 郭阳12郑静2胡愉2崔莲仙2何维2 (湖北医药学院基础医学院免疫教研室湖北十堰442000 2北京协和医学院基础学院中国医学科学院基础医学研究所免疫学系北京100005) 「摘要]目的:研究TCR962(OT3)Fc融合蛋白在体内抗肿瘤效果。方法:通过 同位素标记技术观察TCRγ9/62(0I3}Fc在荷瘤裸鼠体内的显像和分布, 及观察TCRγ9δ2(OT3)-Fc对荷瘤裸鼠肿瘤生长和生存期的影响。结果:在荷瘤 裸鼠体内∞I-TCRγ962(O3)-Fc与肿瘤组织有明显的结合,并且其可抑制荷瘤 裸鼠肿瘤生长速度,延长小鼠生存期。结论:本研究初步证明TCRγ9/62(OT3)Fc 在荷瘤裸鼠体内有一定的抗肿瘤效果,为肿瘤靶向性抗体治疗提供了新的思路 关键词]TCRγ962(OT3}Fc;肿瘤靶向治疗;荷瘤裸鼠 Anti-tumor Effect ofTCry 9/82(OT3)-Fc in Nude Mice Bearing Tumor GUO Yang 2, ZHENG Jing2, HU Yu2, CUI Lian-xian HE Wei? A School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei 442000; Department o Immunology, BMS& School of Basic Medicine, CAMS PUMC, Beijing 100005, China Abstract: Objective To investigate the anti-tumor effects of TCry9/82(OT3)-Fc fusion protein in vivo. Methods We observed the imaging and distribution of TCRy9/82(OT3-Fc in nude mice bearing tumor through labeling 99mTc. Meanwhile we observed the tumor growth and survival of nude mice with the treatment of TCRy 9/82(OT3)-Fc. Results In nude mice 9gmTc-TCRy 9/2(OT3)-Fc showed obvious binding ability with tumor tissue and it inhibited tumor growth in nude mice and prolong surv ival of mice. Conclusion Our preliminary study demonstrated the anti-tumor effect of TCry 9/02(OT3)-Fc in nude mice bearing tumor and provide a novel strategy for tumor targeting antibody therapy Key words: TCRy 9/62(OT3)-Fc, tumor targeting therapy; nude mice bearing tumor [基金项目]国家高技术研究发展计划(863计划)(2006AA02A245) [作者简介]郭阳(1979-),女,湖北文安人,讲师,博士,研究方向:肿瘤免疫学 [通信作者]何维(1955-),男,黑龙江呼兰人,教授,博士生导师,研究方向:肿瘤免 疫学。E-mail:heweiimu@public.bta.net.cn[基金项目] 国家高技术研究发展计划(863 计划)（2006AA02A245） [作者简介] 郭 阳（1979-），女，湖北文安人，讲师，博士，研究方向：肿瘤免疫学。 [通信作者] 何 维（1955-），男，黑龙江呼兰人，教授，博士生导师，研究方向：肿瘤免 疫学。E-mail:heweiimu@public.bta.net.cn TCR9/2(OT3)-Fc 融合蛋白体内抗肿瘤效果研究 郭阳 1,2 郑静 2 胡愉 2 崔莲仙 2 何维 2， （1 湖北医药学院基础医学院免疫教研室 湖北十堰 442000； 2 北京协和医学院基础学院中国医学科学院基础医学研究所免疫学系 北京 100005） [摘要]目的：研究 TCR9/2(OT3)-Fc 融合蛋白在体内抗肿瘤效果。方法：通过 同位素 99mTc 标记技术观察 TCR9/2(OT3)-Fc 在荷瘤裸鼠体内的显像和分布， 及观察 TCR9/2(OT3)-Fc 对荷瘤裸鼠肿瘤生长和生存期的影响。结果：在荷瘤 裸鼠体内 99mTc-TCRγ9/δ2(OT3)-Fc 与肿瘤组织有明显的结合，并且其可抑制荷瘤 裸鼠肿瘤生长速度，延长小鼠生存期。结论：本研究初步证明 TCR9/2(OT3)-Fc 在荷瘤裸鼠体内有一定的抗肿瘤效果，为肿瘤靶向性抗体治疗提供了新的思路。 [关键词] TCR9/2(OT3)-Fc；肿瘤靶向治疗；荷瘤裸鼠 Anti-tumorEffect of TCR9/2(OT3)-Fc in Nude Mice Bearing Tumor GUO Yang1,2, ZHENG Jing2, HU Yu2 , CUI Lian-xian2 , HE Wei2 ( 1 School of Basic Medicine,Hubei University of Medicine,Shiyan,Hubei 442000; 2Department of Immunology,IBMS& School of Basic Medicine,CAMS & PUMC,Beijing 100005,China) Abstract: Objective To investigate the anti-tumor effects of TCR9/2(OT3)-Fc fusion protein in vivo. Methods We observed the imaging and distribution of TCR9/2(OT3)-Fc in nude mice bearing tumor through labeling 99mTc. Meanwhile, we observed the tumor growth and survival of nude mice with the treatment of TCR9/2(OT3)-Fc. Results In nude mice 99mTc-TCR9/2(OT3)-Fc showed obvious binding ability with tumor tissue and it inhibited tumor growth in nude mice and prolong survival of mice. Conclusion Our preliminary study demonstrated the anti-tumor effect of TCR9/2(OT3)-Fc in nude mice bearing tumor and provide a novel strategy for tumor targeting antibody therapy. Key words: TCR9/2(OT3)-Fc; tumor targeting therapy; nude mice bearing tumor