ELEMENTS OF TISSUE ENGINEERING/ REGENERATIVE MEDICINE MATRIX (SCAFFOLD) Porous, absorbable synthetic(e,g, polyglycolic acid)and natural(eg, collagen) biomaterials CELLS (Autologous or Allogeneic Differentiated cells of same type as tissue Stem cells(e.g, bone marrow-derived Other cell types(e.g, dermal cells) SOLUBLE REGULATORS Growth factors or their genes ENVIRONMENTAL FACTORS Mechanical loading Static versus dynamic(“ bioreactor”)ELEMENTS OF TISSUE ENGINEERING/ ELEMENTS OF TISSUE ENGINEERING/ REGENERATIVE MEDICINE REGENERATIVE MEDICINE • MATRIX (SCAFFOLD) MATRIX (SCAFFOLD) –Porous, absorbable synthetic ( Porous, absorbable synthetic (e.g., polyglycolic ., polyglycolic acid) and natural ( acid) and natural (e.g., collagen) biomaterials ., collagen) biomaterials • CELLS (Autologous or Allogeneic) CELLS (Autologous or Allogeneic) –Differentiated cells of same type as tissue Differentiated cells of same type as tissue – Stem cells ( Stem cells (e.g., bone marrow ., bone marrow-derived) derived) –Other cell types ( Other cell types (e.g., dermal cells) ., dermal cells) • SOLUBLE REGULATORS SOLUBLE REGULATORS –Growth factors or their genes Growth factors or their genes • ENVIRONMENTAL FACTORS ENVIRONMENTAL FACTORS –Mechanical loading Mechanical loading – Static versus dynamic (“bioreactor”) Static versus dynamic (“bioreactor”)