华南农业大学学报（自然科学板） 第24卷 p].Seed Science and Techmology,1975,3 (1):161- tion of tomato (Lveopericon esetento L)by ultrathirlaye 169. isoelectrie focusing of seed protein [J ]Seed Science and [RADCLA BJ.Ultrathirlayer isoelectric focusing in 50-100 Techrology,2000.28:201-213. m polyacrylamide gels on silanized glass plates or polyester [6]VANANGAMUDI K,PALANISAMYV,NATESAN P,ct al. films[J ]Hlectrophoresis.1980.1:43-56. Variety determination in riceexamination of the hulled grain xes[J].Seed U1.Seed Science and Technoloay 1988 16:457-464. 99,27. 333 [71 [4]LUCCHESEC.DNBLI G d ele Intemational Rice Re 37.47 [8】何忠效，张树政.电泳M.第2版.北京：科学出版社 [5]WANGXF,KNOBLAUCH R,LEISTN.Varictal discriminer 1999.59-104. Varietal Identification and Genetic Purity Testing of Hybrid Maize,Capsicum and Rice in Laboratory YAN Min,WANG Xiaorfeng (College of Life Science,South China Agric.Univ.Guanghou 510642,China AbstractUtrathilayer iselectric focusing of seed protein was used for varietal identification and genetic purity testing of hybrid maize,capsicum and rice.was found that the Fseeds of hybrid maizeand capsicum1 had both of the male and female marker bands from parental lines,and were considered to be genuine hybrids. hybrid maize(cv.Yueyou 1)and rice(cv.Huaguiyou 99)were found to be not genetically pure. Key maize hybrid rice hybrid capsicum varietal purity 【责任编辑柴焰】 [J ]. Seed Science and Technology , 1975 , 3 (1) : 161 - 169. [2 ] RADOLA B J . Ultrathin2layer isoelectric focusing in 50 - 100 μm polyacrylamide gels on silanized glass plates or polyester films[J ]. Electrophoresis , 1980 , 1 :43 - 56. [3 ] ISTA. International rules for seed testing2Annexes[J ]. Seed Science and Technology , 1999 , 27 , Supplement :1 - 333. [4 ] LUCCHESE C , DINELLI G, MIGGIANO A ,et al. Identifica2 tion of pepper ( Capsicum spp. ) cultivars by field and elec2 trophoresis tests[J ]. Seed Science and Technology ,1999 ,27 : 37 - 47. [ 5 ] WANG X F , KNOBLAUCH R , LEIST N. Varietal discrimina2 tion of tomato ( Lycopersicon esculentum L) by ultrathin2layer isoelectric focusing of seed protein [J ]. Seed Science and Technology , 2000 , 28 : 201 - 213. [6 ] VANANGAMUDI K, PALANISAMY V , NATESAN P , et al. Variety determination in rice2examination of the hulled grain [J ]. Seed Science and Technology , 1988 , 16 : 457 - 464. [7 ] WANG X F , KNOBLAUCH R , LEIST N. Identification of va2 rieties and testing of hybrid purity of rice by ultrathin2layer iso2 electric focusing of seed protein [J ]. International Rice Re2 search Notes , 2001 , 26(1) : 18 - 19. [8 ] 何忠效 ,张树政. 电泳[M]. 第 2 版. 北京 :科学出版社 , 1999. 59 - 104. Varietal Identification and Genetic Purity Testing of Hybrid Maize , Capsicum and Rice in Laboratory YAN Min , WANG Xiao2feng (College of Life Science , South China Agric. Univ. , Guangzhou 510642 , China) Abstract :Ultrathin2layer isoelectric focusing of seed protein was used for varietal identification and genetic purity testing of hybrid maize , capsicum and rice. It was found that the F1 seeds of hybrid maize 8 ×9 and capsicum 1 ×3 had both of the male and female marker bands from parental lines , and were considered to be genuine hybrids. However , F1 seeds of hybrid maize (cv. Yueyou 1) and rice (cv. Huaguiyou 99) were found to be not genetically pure. Key words :hybrid maize ; hybrid rice ; hybrid capsicum ; varietal identification ; genetic purity 【责任编辑 柴 焰】 8 华 南 农 业 大 学 学 报 (自 然 科 学 版) 第 24 卷 © 1995-2005 Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved