pharmacological activities have been identified as the development of biological analysis, these components constitute a substantial percentage of todays new drugs#4. One of the most commonly used method to investigate new drugs is to start from a disease and search from natural products which could treat it, however, the limitation of technical difficulties and suitable animal models makes it difficult to understand their mode of action 5,46. Recently, systems pharmacology offers a new approach to study bioactive components of TCM agents, as well as their drug-target interactions. Herein, we try to selective adjuvant from O. sinensis for its remarkable immunoregulation effects. Although there have been many studies about effective components in O. sinensis, the exact action mode was not fully elicited at a system level In the present study, with the help of OB, DL and HL screening, 17 active compounds with 190 potential targets were identified in O. sinensis. The target enrichment analysis shows that these targets take part in many biological pathways such as cytokine secretion, drug binding, blood circulation and so on. Several targets were lso involved in different types of cancer, supporting an earlier study which showed O sinensis extracts to exhibit anti-cancer effects"-49. It was not a surprise that several ts have been studied clearly in ches. Caffeine is well-known phosphodiesterase 3(PDE3)inhibitor and antagonist of adenosine receptors. Caffeine can inhibit the production of tumor necrosis factor alpha(tNF-a in lipopolysaccharide-stimulated human whole blood and modulate specific biological parameters associated with depression, thereby preventing the disease 2. 539 pharmacological activities have been identified as the development of biological analysis; these components constitute a substantial percentage of today’s new drugs43,44. One of the most commonly used method to investigate new drugs is to start from a disease and search from natural products which could treat it, however, the limitation of technical difficulties and suitable animal models makes it difficult to understand their mode of action45,46. Recently, systems pharmacology offers a new approach to study bioactive components of TCM agents, as well as their drug-target interactions. Herein, we try to selective adjuvant from O. sinensis for its remarkable immunoregulation effects. Although there have been many studies about effective components in O. sinensis, the exact action mode was not fully elicited at a system level. In the present study, with the help of OB, DL and HL screening, 17 active compounds with 190 potential targets were identified in O. sinensis. The target enrichment analysis shows that these targets take part in many biological pathways such as cytokine secretion, drug binding, blood circulation and so on. Several targets were also involved in different types of cancer, supporting an earlier study which showed O. sinensis extracts to exhibit anti-cancer effects47-49. It was not a surprise that several major components have been studied clearly in previous researches. Caffeine is a well-known phosphodiesterase 3 (PDE3) inhibitor and antagonist of adenosine receptors50. Caffeine can inhibit the production of tumor necrosis factor alpha (TNF-α) in lipopolysaccharide-stimulated human whole blood51 and modulate specific biological parameters associated with depression, thereby preventing the disease52,53