Interestingly, caffeine can also protect against the production of free radicals 4. Sito is a functional phytosterol with a chemical structure similar to that of cholesterol. Sito alone or combined with other plant sterols is known to reduce blood cholesterol levels by blocking cholesterol absorption 6,7 Sito has also been reported to possess antimicrobial activity". AA, an unsaturated fatty acid that modulates the activities of various ion channels, functions as a second messenger. As the adjuvant selection purpose, we choose all immune related function to get a component-target -function network. Results show that all components participate in these processes. Then,an immune-related pathway was constructed to further dissect the potential immunoregulation activities of components in O. sinensis. Cordycepin was finally selected because of its significant functions to suppress inflammation and regulate leukocyte migration as well as cell proliferation in the networks. Although it was not the only key component in O. sinensis, other components may interference with nutrient absorption or inhibit cytokine secretion, may have an adverse effect on healthy people or just suppress immune responses There have been many studies on cordycepin these days, mainly concentrate on its anti-tumor and immunoregulation effects, but few reports pay attention to its adjuvanticity to vaccines In the experimental validation part, cordycepin triggered IgM and Igg antibody responses in mice by 7 days in a dose dependent manner after combination with a hepatitis b vaccine. The high cordycepin group showed no difference in IgG titers between days 14 and 21 suggesting that the third injection might be unnecessary. Certainly, this would reduce the overall cost and the immune10 Interestingly, caffeine can also protect against the production of free radicals54. Sito is a functional phytosterol with a chemical structure similar to that of cholesterol55. Sito alone or combined with other plant sterols is known to reduce blood cholesterol levels by blocking cholesterol absorption56,57. Sito has also been reported to possess antimicrobial activity58. AA, an unsaturated fatty acid that modulates the activities of various ion channels, functions as a second messenger59. As the adjuvant selection purpose, we choose all immune related function to get a component-target-function network. Results show that all components participate in these processes. Then, an immune-related pathway was constructed to further dissect the potential immunoregulation activities of components in O. sinensis. Cordycepin was finally selected because of its significant functions to suppress inflammation and regulate leukocyte migration as well as cell proliferation in the networks. Although it was not the only key component in O. sinensis, other components may interference with nutrient absorption or inhibit cytokine secretion, may have an adverse effect on healthy people or just suppress immune responses. There have been many studies on cordycepin these days, mainly concentrate on its anti-tumor and immunoregulation effects60-62, but few reports pay attention to its adjuvanticity to vaccines. In the experimental validation part, cordycepin triggered IgM and IgG antibody responses in mice by 7 days in a dose dependent manner after combination with a hepatitis b vaccine. The high cordycepin group showed no difference in IgG titers between days 14 and 21 suggesting that the third injection might be unnecessary. Certainly, this would reduce the overall cost and the immune