response time. Moreover, primary safety evaluation demonstrates that a high dose of cordycepin adjuvanted with HBsAg accelerated antibody production more rapidly than the existing vaccine without any side effects Enhancement of celluar immunity is also important for defense of HBV infection especially T cell responses and T cell related cytokines which involved in B cell activation and antibody production. The Thl-type of cellular immune response damages the virus, whereas the main function of the Th2-type of cellular immune response is to promote B cell production of antibodies. IFN-y is a multifunctional cytokine that is primarily produced by Thl cells, CTLs and natural killer cells with antiviral and immunomodulatory activities. IL-4 controls Th2 differentiation and plays an important role in antibody class switching.68. Enhanced cytokine production by cordycepin suggests that it accelerates Thl and Th2 cell responses at the same time Jeong et al. also demonstrated that cordycepin increases the levels of different cytokines in vitro, contributing to the enhancement of immunity. The Th2 cell activation results in b cell maturation which consistent with the b cell classification experiments To sum it up, the systems pharmacology analysis offers us a new way to discover effective adjuvants from certain TCMs. Here we get a comprehensive understanding of function mechanism of Ophiocordyceps sinensis in a molecular-target level In the component-target network, the active components showed enormous polypharmacology, and after pathway enrichment analysis, multiple targets were found to participate in many physiological activities, including ion transport and11 response time. Moreover, primary safety evaluation demonstrates that a high dose of cordycepin adjuvanted with HBsAg accelerated antibody production more rapidly than the existing vaccine without any side effects. Enhancement of celluar immunity is also important for defense of HBV infection, especially T cell responses and T cell related cytokines which involved in B cell activation and antibody production63. The Th1-type of cellular immune response damages the virus, whereas the main function of the Th2-type of cellular immune response is to promote B cell production of antibodies. IFN-γ is a multifunctional cytokine that is primarily produced by Th1 cells, CTLs and natural killer cells with antiviral and immunomodulatory activities64-66. IL-4 controls Th2 differentiation and plays an important role in antibody class switching67,68. Enhanced cytokine production by cordycepin suggests that it accelerates Th1 and Th2 cell responses at the same time. Jeong et al. also demonstrated that cordycepin increases the levels of different cytokines in vitro, contributing to the enhancement of immunity69. The Th2 cell activation results in B cell maturation which consistent with the B cell classification experiments. To sum it up, the systems pharmacology analysis offers us a new way to discover effective adjuvants from certain TCMs. Here we get a comprehensive understanding of function mechanism of Ophiocordyceps sinensis in a molecular-target level. In the component-target network, the active components showed enormous polypharmacology, and after pathway enrichment analysis, multiple targets were found to participate in many physiological activities, including ion transport and