Designer Monoclonal Antibodies mice After Five Days emove Spleen ◆ CDNA Restriction ..CCA Enzymes Digestion 品 and Ligated Digestion Inserted Restriction Enzymes 124 Transfer to E.coli Detect clones by an antigen Ahi6ooging The mRNA is isolated from B cells in the spleen and used to produce complementary (DNA).The DNA sequence for Ig,H and L chains,is amplified by polymerase chain reaction (PCR)using primers.The H and L chain gene products are then digested with restriction enzymes into small DNA fragments.These are then inserted(ligated)into viral DNA of a phage and the phage is used to infect E.coli.The virus grows lytically and antibody chains can be detected with antigens. ReturnThe mRNA is isolated from B cells in the spleen and used to produce complementary (DNA). The DNA sequence for Ig, H and L chains, is amplified by polymerase chain reaction (PCR) using primers. The H and L chain gene products are then digested with restriction enzymes into small DNA fragments. These are then inserted (ligated) into viral DNA of a phage and the phage is used to infect E. coli. The virus grows lytically and antibody chains can be detected with antigens. Return