HEMOLYTIC PLAQUE ASSAY Remove spleer Add lymphocyte 5 days Pour on slide and incubate 45 min. Non secreting cells CompTement 9 Antibodies plus Hybridomas In 1960,a spontaneous cell fusion was observed and,in 1973,Cotton and Milstein and rat IgG molecules ingenious method for the production of monoclonal antibodies by fusion of myeloma cells with spleen cells from an immunized mouse. Designer Monoclonal Antibodies Hybridomas In 1960, a spontaneous cell fusion was observed and, in 1973, Cotton and Milstein fused mouse and rat myeloma cells, forming hybrids capable of secreting both mouse and rat IgG molecules. This was the beginning of cell fusion and lead to tlhe production of monoclonal antibodies. In 1975 Kohler and Milstein provided an ingenious method for the production of monoclonal antibodies by fusion of myeloma cells with spleen cells from an immunized mouse. Designer Monoclonal Antibodies