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Vol.29 Suppl.2 邱丽娜等：一株硫氧化菌的筛选与表征 215. 参考文献 cal reactions in enhancement of rate of leaching.Chem Eng Sci. 2004.59.5069 [1]周顺贵，王世梅，余素萍，等.污泥中氧化亚铁硫杆菌的分离及 其应用效果.环境科学，2003,24(3)：56 [7]周顺桂，周立祥，王世梅。嗜酸硫氧化菌株的分离及其在污泥 生物脱毒中的应用.环境科学研究，2003.16(5)：41 [2]Kumari A.Natarajan K A.Electrobioleaching of polymetallic o- cean nodules.Hydrometallurgy.2001,62(10):125 [8】周立祥，王艮梅.污水污泥中重金属的细菌淋滤效果研究·环 [3]沈镭，张太平，贾晓珊。利用氧化亚铁硫杆菌和氧化硫硫杆菌 境科学学报，2001,21(4).504 去除污泥中重金属的研究，中山大学学报（自然科学版）， [9]歌冰，郑宇，邸进申.氧化亚铁硫杆菌的生物学特性研究进展 2005,44(2):111 生物技术，2004,14(2)：71 [4]朱南文，蔡春光，吴志超，等.污泥中重金属的生物沥滤及其机 [10]HosseiniT R.Kolahdoozan M.Tabatabaei Y S M.Bioflotation 理分析.上海交通大学学报，2003,37(5)：801 of Sarcheshmeh copper are using Thiobacillus Ferrooxidans bac- [5]Jaworska M,Urbanek A.The influence of oxygen concentration teria.Miner Eng.2005,18(3):371 [11]钱存柔，黄仪秀，微生物实验教程，北京：北京大学出版社， in liquid medium on elemental sulphur oxidation by Thiobacillus 1999 thiooxidans.Bioprocess Eng.1998.18:201 [6]Kodali B.Rao M B.Narasu M L.Pogaku R.Effect of biochemi- Isolation and characterization of a sulfur oxidizing bacterium QIU Lina,LI Yuanyuan,GONG Aijun,YAN Hai,WANG Xiaoning Applied Science School.University of Science and Technology Beijing.Beijing 100083.China ABSTRACI A sulfur-oxidizing bacterial strain was isolated from sew age sludge using liquid selective medium and then purified on solid selective medium.The bacteriam can metabolize sofur or S2O to SOi during growth.The sulfur-oxidizing strain is Gram-negative,and has ability to swim.After three days of cultivation, the results showed that the pH of medium decreased.After one week,the pH of the medium dropped to about 0.6,which suggests sulfur oxidizion is taken place.The concentration of the sulfate was tested by using ion chromatography,and the results showed that the concentration of the sulfate increased from 249.2mgLto3 679.7mgL.The sulfur oxidizing bacterium was then inoculated into the FeS medium,as expected,the FeS was oxidized and the media became lighter in color. KEY WORDS sulfur-oxidizing bacterium:isolation:characterization参 考 文 献 ［1］ 周顺贵王世梅余素萍等．污泥中氧化亚铁硫杆菌的分离及 其应用效果．环境科学200324（3）：56 ［2］ Kumari ANatarajan K A．Electrobioleaching of polymetallic ocean nodules．Hydrometallurgy200162（10）：125 ［3］ 沈镭张太平贾晓珊．利用氧化亚铁硫杆菌和氧化硫硫杆菌 去除污泥中重金属的研究．中山大学学报（自然科学版） 200544（2）：111 ［4］ 朱南文蔡春光吴志超等．污泥中重金属的生物沥滤及其机 理分析．上海交通大学学报200337（5）：801 ［5］ Jaworska MUrbanek A．The influence of oxygen concentration in liquid medium on elemental sulphur oxidation by Thiobacillus thiooxidans．Bioprocess Eng199818：201 ［6］ Kodali BRao M BNarasu M LPogaku R．Effect of biochemical reactions in enhancement of rate of leaching．Chem Eng Sci 200459：5069 ［7］ 周顺桂周立祥王世梅．嗜酸硫氧化菌株的分离及其在污泥 生物脱毒中的应用．环境科学研究200316（5）：41 ［8］ 周立祥王艮梅．污水污泥中重金属的细菌淋滤效果研究．环 境科学学报200121（4）：504 ［9］ 耿冰郑宇邸进申．氧化亚铁硫杆菌的生物学特性研究进展． 生物技术200414（2）：71 ［10］ Hosseini T RKolahdoozan MTabatabaei Y S M．Bioflotation of Sarcheshmeh copper are using Thiobacillus Ferrooxidans bacteria．Miner Eng200518（3）：371 ［11］ 钱存柔黄仪秀．微生物实验教程．北京：北京大学出版社 1999 Isolation and characterization of a sulfur-oxidizing bacterium QIU L inaLI Y uanyuanGONG A ijunY A N HaiWA NG Xiaoning Applied Science SchoolUniversity of Science and Technology BeijingBeijing100083China ABSTRACT A sulfur-oxidizing bacterial strain was isolated from sewage sludge using liquid selective medium and then purified on solid selective medium．The bacteriam can metabolize sofur or S2O 2－ 3 to SO 2－ 4 during growth．The sulfur-oxidizing strain is Gram-negativeand has ability to swim．After three days of cultivation the results showed that the pH of medium decreased．After one weekthe pH of the medium dropped to about 0∙6which suggests sulfur oxidizion is taken place．The concentration of the sulfate was tested by using ion chromatographyand the results showed that the concentration of the sulfate increased from249∙2mg·L －1 to3 679∙7mg·L －1．The sulfur-oxidizing bacterium was then inoculated into the FeS mediumas expectedthe FeS was oxidized and the media became lighter in color． KEY WORDS sulfur-oxidizing bacterium；isolation；characterization Vol．29Suppl．2 邱丽娜等： 一株硫氧化菌的筛选与表征 ·215·