.770. 北京科技大学学报 第29卷 Heterotrophic culture of Chlorella sp USTBOl and production of lutein WANG Suqin2),LI Yawen),YAN Hai,YANG Shuai2),LIN Hai?) 1)Applied Science School.University of Science and Technology Beijing.Beijing 100083.China 2)Civil and Environmental Engineering School,University of Science and Technology Beijing.Beijing 100083,China 3)Department of Biological Technology,Beijing University of Aeronautics and Astronautics.Beijing 100083.China ABSTRACI The effects of carbon and nitrogen sources as well as carbon to-nitrogen mass ratio on the growth of Chlorella sp USTBOl and the production of lutein were investigated under the condition of heterotrophic cul- ture in dark.The results show that glucose and potassium nitrate were the best carbon and nitrogen sources to support the rapid and continuous growth of Chlorella sp USTBO1 respectively.When glucose and potassium ni- trate were used as the sole carbon and nitrogen sources and the initial concentration of nitrogen was.8g the growth of Chlorella sp increased with the increase of carbon to-nitrogen mass ratio in the range from 15 to 25.Potassium nitrate was the best nitrogen source to promote biosynthesis of lutein by Chlorella sp USTBO1 and the content of lutein in the cells of Chlorella sp USTBO1 declined with the increase of carbon-nitrogen mass ratio from 15 to 30. KEY WORDS Chlorella sp:heterotrophic culture;lutein;carbon source;nitrogen source;carbon to nitrogen ratio (上接第758页) Influence factors of mine gas hydrate formation in surfactant solution containing coal WU Qiang,ZHA NG Baoyong Safety Engineering Technology School,Heilongjiang Institute of Science Technology.Harbin 150027,China ABSTRACI In order to promote mine gas hydrate formation quickly,which can prevent coal and gas outburst, the processes of gas hydrate formation in three reaction systems containing coal were investigated by utilizing the visible experimental installation under the condition of 14.3-25.4C and 18.66-26.70 MPa,and the correla- tions among pressure,temperature and time were obtained.Experimental data were analyzed based on the com- putation models of formation rate and volumetric proportion.The results show that the addition of surfactant shortens induction time and improves formation rate and volumetric proportion,and the volumetric proportion of synthetic hydrate samples can amount to 136-150;the existence of porous medium (coal)delays hydrate for- mation exiguously;the influence of memory effect on gas hydrate formation is notable,the induction time could be shortened by 10-20 times for the experimental system containing residual pentahedral ring structure. KEY WORDS mine gas:hydrate;surfactant;formation rate;memory effect;influence factorHeterotrophic culture of Chlorella sp USTB01and production of lutein WA NG Suqin 1‚2）‚LI Y awen 3）‚Y A N Hai 1）‚Y A NG Shuai 2）‚LIN Hai 2） 1） Applied Science School‚University of Science and Technology Beijing‚Beijing100083‚China 2） Civil and Environmental Engineering School‚University of Science and Technology Beijing‚Beijing100083‚China 3） Department of Biological Technology‚Beijing University of Aeronautics and Astronautics‚Beijing100083‚China ABSTRACT The effects of carbon and nitrogen sources as well as carbon-to-nitrogen mass ratio on the growth of Chlorella sp USTB01and the production of lutein were investigated under the condition of heterotrophic cul￾ture in dark．The results show that glucose and potassium nitrate were the best carbon and nitrogen sources to support the rapid and continuous growth of Chlorella sp USTB01respectively．When glucose and potassium ni￾trate were used as the sole carbon and nitrogen sources and the initial concentration of nitrogen was0∙28g·L －1‚ the growth of Chlorella sp increased with the increase of carbon-to-nitrogen mass ratio in the range from 15to 25．Potassium nitrate was the best nitrogen source to promote biosynthesis of lutein by Chlorella sp USTB01 and the content of lutein in the cells of Chlorella sp USTB01declined with the increase of carbon-nitrogen mass ratio from15to30． KEY WORDS Chlorella sp；heterotrophic culture；lutein；carbon source；nitrogen source；carbon-to-nitrogen ratio （上接第758页） Influence factors of mine gas hydrate formation in surfactant solution containing coal W U Qiang‚ZHA NG Baoyong Safety Engineering ＆ Technology School‚Heilongjiang Institute of Science ＆ Technology‚Harbin150027‚China ABSTRACT In order to promote mine gas hydrate formation quickly‚which can prevent coal and gas outburst‚ the processes of gas hydrate formation in three reaction systems containing coal were investigated by utilizing the visible experimental installation under the condition of14∙3－25∙4℃ and18∙66－26∙70MPa‚and the correla￾tions among pressure‚temperature and time were obtained．Experimental data were analyzed based on the com￾putation models of formation rate and volumetric proportion．The results show that the addition of surfactant shortens induction time and improves formation rate and volumetric proportion‚and the volumetric proportion of synthetic hydrate samples can amount to136－150；the existence of porous medium （coal） delays hydrate for￾mation exiguously；the influence of memory effect on gas hydrate formation is notable‚the induction time could be shortened by 10－20times for the experimental system containing residual pentahedral ring structure． KEY WORDS mine gas；hydrate；surfactant；formation rate；memory effect；influence factor ·770· 北 京 科 技 大 学 学 报 第29卷