南京农业大学学报2016,39(3)：366-372 http://nauxb.njau.edu.cn Journal of Nanjing Agricultural University D0L:10.7685/jmau.201510023 崔红米，曹学伟，王建军，等.外源GR24对不结球白菜腋芽生长的影响[J].南京农业大学学报，2016,39(3)：366-372 外源GR24对不结球白菜腋芽生长的影响 崔红米，曹学伟，王建军，熊爱生，侯喜林，李英 (南京农业大学作物遗传与种质创新国家重点实验室，江苏南京210095) 摘要：[目的]本文旨在探究独脚金内酯对不结球白菜腋芽生长的影响。[方法]采用不同浓度的独脚金内酯人工类似物 GR24处理不结球白菜‘如皋毛菜'，调查腋芽表型的变化，采用超高效液相色谱(UPLC)法测定叶腋处细胞分裂素(Z+ZR) 和生长素(IAA)的含量，用荧光定量PCR检测叶腋处分蘖/分枝相关基因的表达。[结果]外源GR24能抑制不结球白菜腋 芽的萌发，且浓度越高抑制作用越明显，同时还能抑制腋芽的伸长，但低浓度GR24的抑制作用更明显。外源GR24处理 后，不结球白菜叶腋处的Z+ZR和IAA含量显著降低，且低浓度的GR24发挥作用快。外源GR24能促进叶腋处独脚金内 酯响应基因MAK2和BRC1的表达，且MAX2的表达量变化比BRC1的变化早：外源GR24能抑制细胞分裂素合成相关基因 LOG1的表达：外源GR24处理后，抑制腋生分生组织形成与生长的基因SPS和抑制新叶形成的基因SPL9的表达量都明显 升高。[结论]外源G24能直接抑制不结球白菜腋芽的萌发和伸长，也能通过调控其他激素相关信号和分蘗/分枝相关基 因的表达来间接抑制不结球白菜腋芽的萌发和伸长。 关键词：不结球白菜：独脚金内酯：腋芽：基因表达 中图分类号：S634.3 文献标志码：A 文章编号：1000-2030(2016)03-0366-07 Effects of exogenous GR24 on the growth of axillary bud of non -heading Chinese cabbage CUI Hongmi,CAO Xuewei,WANG Jianjun,XIONG Aisheng,HOU Xilin,LI Ying" (State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China) Abstract [Objectives]The effects of strigolactones on axillary bud growth was researched in non-heading Chinese cabbage [Methods]GR24(artificial analogue of strigolactones)at different concentrations were applied to the roots of non-heading Chinese cabbage 'Rugaomaocai'.The number and total length of active buds were measured;the contents of cytokinins(Z+ZR)and auxin (IAA)in non-heading Chinese cabbage leaf axil were determined using UPLC(ultra performance liquid chromatography),and the relative expression of tillering/branching related genes in non-heading Chinese cabbage leaf axil was tested by RT-PCR.[Results] As a result,exogenous GR24 inhibited axillary bud of non-heading Chinese cabbage releasing from dormancy and the effect of higher concentration of GR24 was stronger.While lower concentration of GR24 could inhibit axillary bud elongation more than higher concentration.After GR24 application,the contents of Z+ZR and IAA were lower than control.While lower concentration of GR24 effected faster.Compared to the control,the relative expression of strigolactones perception gene MAY2 and BRCI increased after GR24 appliction.In addition,the relative expression of MAY2 changed earlier than BRCI.After GR24 application,the relative expression of cytokinins related gene LOG/was lower than control.The relative expression of SPS(regulating the formation and growth of axillary meristem negatively)and SPL9(inhibiting the formation of new leaf)increased after GR24 application. [Conclusions]Exogenous GR24 can inhibit axillary bud releasing from dormancy and axillary bud elongation of non-heading Chinese cabbage directly.At the same time,exogenous GR24 can inhibit axillary bud releasing from dormancy and axillary bud elongation indirectly by regulating the hormones signals and expression of tillerring/branching related genes. Keywords:non-heading Chinese cabbage;strigolactones;axillary bud;gene expression 独脚金内酯(strigolactones.,SLs)是一种产生于植物根部的类胡萝卜衍生物，最早是作为寄生植物种子 萌发刺激物而发现。近期研究发现，该类化合物能够抑制植物的分枝，是继生长素和细胞分裂素之后发现 的调控植物分枝的新型植物激素1-)。GR24为独脚金内酯的人工类似物，现己应用于水稻、豌豆、拟南芥 收稿日期：2015-10-17 基金项目：江苏高校优势学科建设工程项目：国家863计划项目子课题(2012AA100202-8):江苏省科技支撑计划项目(BE2012325):江苏省 杰出青年基金项目(BK20130027) 作者简介：崔红米，硕士研究生.”通信作者：李英，教授，博导，研究方向为蔬菜遗传育种与分子生物学，Tel:025-84395756,Eai让：yingli@ njau.cdu.cn。 ?1994-2016 China Academic Journal Electronic Publishing House.All rights reserved.http://www.cnki.net南京农业大学学报 2016，39( 3) : 366－372 http: / / nauxb．njau．edu．cn Journal of Nanjing Agricultural University DOI: 10．7685/jnau．201510023  收稿日期: 2015－10－17 基金项目: 江苏高校优势学科建设工程项目; 国家 863 计划项目子课题( 2012AA100202－8) ; 江苏省科技支撑计划项目( BE2012325) ; 江苏省 杰出青年基金项目( BK20130027) 作者简介: 崔红米，硕士研究生。* 通信作者: 李英，教授，博导，研究方向为蔬菜遗传育种与分子生物学，Tel: 025－84395756，E-mail: yingli@ njau．edu．cn。 崔红米，曹学伟，王建军，等． 外源 GＲ24 对不结球白菜腋芽生长的影响［J］． 南京农业大学学报，2016，39( 3) : 366－372． 外源 GＲ24 对不结球白菜腋芽生长的影响 崔红米，曹学伟，王建军，熊爱生，侯喜林，李英* ( 南京农业大学作物遗传与种质创新国家重点实验室，江苏 南京 210095) 摘要:［目的］本文旨在探究独脚金内酯对不结球白菜腋芽生长的影响。［方法］采用不同浓度的独脚金内酯人工类似物 GＲ24 处理不结球白菜‘如皋毛菜’，调查腋芽表型的变化，采用超高效液相色谱( UPLC) 法测定叶腋处细胞分裂素( Z+ZＲ) 和生长素( IAA) 的含量，用荧光定量 PCＲ 检测叶腋处分蘖/分枝相关基因的表达。［结果］外源 GＲ24 能抑制不结球白菜腋 芽的萌发，且浓度越高抑制作用越明显，同时还能抑制腋芽的伸长，但低浓度 GＲ24 的抑制作用更明显。外源 GＲ24 处理 后，不结球白菜叶腋处的 Z+ZＲ 和 IAA 含量显著降低，且低浓度的 GＲ24 发挥作用快。外源 GＲ24 能促进叶腋处独脚金内 酯响应基因 MAX2 和 BＲC1 的表达，且 MAX2 的表达量变化比 BＲC1 的变化早; 外源 GＲ24 能抑制细胞分裂素合成相关基因 LOG1 的表达; 外源 GＲ24 处理后，抑制腋生分生组织形成与生长的基因 SPS 和抑制新叶形成的基因 SPL9 的表达量都明显 升高。［结论］外源 GＲ24 能直接抑制不结球白菜腋芽的萌发和伸长，也能通过调控其他激素相关信号和分蘖/分枝相关基 因的表达来间接抑制不结球白菜腋芽的萌发和伸长。 关键词: 不结球白菜; 独脚金内酯; 腋芽; 基因表达 中图分类号: S634．3 文献标志码: A 文章编号: 1000－2030( 2016) 03－0366－07 Effects of exogenous GＲ24 on the growth of axillary bud of non - heading Chinese cabbage CUI Hongmi，CAO Xuewei，WANG Jianjun，XIONG Aisheng，HOU Xilin，LI Ying* ( State Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjing Agricultural University，Nanjing 210095，China) Abstract: ［Objectives］The effects of strigolactones on axillary bud growth was researched in non-heading Chinese cabbage． ［Methods］GＲ24( artificial analogue of strigolactones) at different concentrations were applied to the roots of non-heading Chinese cabbage‘Ｒugaomaocai’． The number and total length of active buds were measured; the contents of cytokinins( Z+ZＲ) and auxin ( IAA) in non-heading Chinese cabbage leaf axil were determined using UPLC( ultra performance liquid chromatography) ，and the relative expression of tillering / branching related genes in non-heading Chinese cabbage leaf axil was tested by ＲT-PCＲ． ［Ｒesults］ As a result，exogenous GＲ24 inhibited axillary bud of non-heading Chinese cabbage releasing from dormancy and the effect of higher concentration of GＲ24 was stronger． While lower concentration of GＲ24 could inhibit axillary bud elongation more than higher concentration． After GＲ24 application，the contents of Z+ZＲ and IAA were lower than control． While lower concentration of GＲ24 effected faster． Compared to the control，the relative expression of strigolactones perception gene MAX2 and BＲC1 increased after GＲ24 appliction． In addition，the relative expression of MAX2 changed earlier than BＲC1． After GＲ24 application，the relative expression of cytokinins related gene LOG1 was lower than control． The relative expression of SPS ( regulating the formation and growth of axillary meristem negatively ) and SPL9 ( inhibiting the formation of new leaf ) increased after GＲ24 application． ［Conclusions］Exogenous GＲ24 can inhibit axillary bud releasing from dormancy and axillary bud elongation of non-heading Chinese cabbage directly． At the same time，exogenous GＲ24 can inhibit axillary bud releasing from dormancy and axillary bud elongation indirectly by regulating the hormones signals and expression of tillerring / branching related genes． Keywords: non-heading Chinese cabbage; strigolactones; axillary bud; gene expression 独脚金内酯( strigolactones，SLs) 是一种产生于植物根部的类胡萝卜衍生物，最早是作为寄生植物种子 萌发刺激物而发现。近期研究发现，该类化合物能够抑制植物的分枝，是继生长素和细胞分裂素之后发现 的调控植物分枝的新型植物激素［1－3］。GＲ24 为独脚金内酯的人工类似物，现已应用于水稻、豌豆、拟南芥