BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 图 E图配器函图断图配肠 ATP-driv 函医 园医 Fig. 2. Image sequence(viewed left to right)of nanopropellers being rotated anticlockwise at 8.3 oil immersion or 60x water immersion and were captured with a CCD video camera(frame rate 30 Hz) The rotational velocity ranged from -0.8 to 8.3 rps, depending on propeller maintained in the flow cell These sequences can be viewed as movies at the Nanoscale Biological Creating motors chemical on/off switch 3 o Mutate binding face of ATPase to contain a 3-amino acid Zinc ion binding domain( 3 histidines o Mutant binds zinc and zinc blocks action of motor Classical allosteric enzyme inhibition o Chelation of zinc returns motor to active state motor: Sitivity of la Higwre2slea 和[k小5m20 w cTmnodeolsrce Turning nanomotor on and off by addition of zn++ and ta a zn*+ chelator. Assembling these hybrid proteins into molecular devices Lecture 13- Hybrid macromolecules 11 of 13BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 QuickTime™ and a GIF decompressor are needed to see this picture. ATP-driven motors ƒ Creating motors with a chemical on/off switch13 o Mutated ATP binding face of ATPase to contain a 3-amino acid Zinc ion binding domain (3 histidines) o Mutant protein binds zinc and zinc blocks action of motor ƒ Classical allosteric enzyme inhibition o Chelation of zinc returns motor to active state Turning nanorotors addition of Zn++ and Zn++ sensitivity of motor: on and off by a Zn++ chelator: ƒ Assembling these hybrid proteins into molecular devices Lecture 13 – Hybrid macromolecules 11 of 13