cluster of gene segments capable of encoding V regions separated from one or more genes capable of encoding C regions. Chain Class Human Mouse Kappa 2 16 Lambda 22 6 H chain 14 12 Rearrangement of v and C Genes During Differentiation Mouse Embryo Cells Adult Mouse Mueloma cells ●● DNA Extracted. 2 .Restriction Endonucleases V 业业 11 听 Incubate with Ig mRNA ompryonic Hyeloma 6raiv/ Hybridization experiments revealed that V region and C region gene segments were separately encoded and located on distinct DNA fragments in embryonic cells,but on the same fragment in mature plasma cells.These studies suggested that DNA rearrangements must have occurred during maturation and differentiation of B lymphocytes.These rearranged DNA segments were destined to be expressed as specific monospecific eptors by antibody forming plasma cells.From the collection of gen segments available in each family,several may be specifically joined to encode an cluster of gene segments capable of encoding V regions separated from one or more genes capable of encoding C regions. ______________________________________ Chain Class Human Mouse ______________________________________ Kappa 2 16 Lambda 22 6 H chain 14 12 ______________________________________ Hybridization experiments revealed that V region and C region gene segments were separately encoded and located on distinct DNA fragments in embryonic cells, but on the same fragment in mature plasma cells. These studies suggested that DNA rearrangements must have occurred during maturation and differentiation of B lymphocytes. These rearranged DNA segments were destined to be expressed as specific monospecific receptors by antibody forming plasma cells. From the collection of gene segments available in each family, several may be specifically joined to encode an