entire antibody chain L or H.It should be note that genes of one family were never intermingled with genes of either of the other families. Extracted germline DNA from mouse embryos and differentiated DNA from myeloma cells were cleaved with restriction enzymes.separated, denatured to yield single-stranded fragments,and extracted.Fragments were hybridized with kappa chain radiolabled probe (mRNA),derived from myeloma cells. The entire mRNA molecule (encoding the V and C regions)or only the 3'half of mRNA (encoding the C region)was employed.Results showed that mRNA for IgL chain hybridized to germline DNA by its Vregion to one DNA fragm to a totally separate DNA fragment.In contrast,the same mRNA hybridized to both V and C regions in a single DNA fragment from fully differentiated cells. Cloning of Specific Ig gene Mouse Myeloma Cell Bacteria (o ve DNA and restriction inte 85 and k With the advent of recombinant DNA technology,it became possible to clone specific antibody genes.Sufficient quantities of specific DNA segments could be obtained, permitting analysis of their organization.Studies of DNA sequence showed that discontinuous segments existed that did not code for peptides. Some noncoding segments were deleted from the DNA before transcription and others from the mRNA after transcription.entire antibody chain L or H. It should be note that genes of one family were never intermingled with genes of either of the other families. Extracted germline DNA from mouse embryos and differentiated DNA from myeloma cells were cleaved with restriction enzymes. Fragments were separated by electrophoresis, denatured to yield single-stranded fragments, and extracted. Fragments were hybridized with kappa chain radiolabled probe (mRNA), derived from myeloma cells. The entire mRNA molecule (encoding the V and C regions) or only the 3' half of mRNA (encoding the C region) was employed. Results showed that mRNA for Ig L chain hybridized to germline DNA by its V region to one DNA fragment and by its C region to a totally separate DNA fragment. In contrast, the same mRNA hybridized to both V and C regions in a single DNA fragment from fully differentiated cells. With the advent of recombinant DNA technology, it became possible to clone specific antibody genes. Sufficient quantities of specific DNA segments could be obtained, permitting analysis of their organization. Studies of DNA sequence showed that discontinuous segments existed that did not code for peptides. Some noncoding segments were deleted from the DNA before transcription and others from the mRNA after transcription