Correlative Light and Electron Microscopy (CLEM) In an integrated CLEM system,the sample is imaged using an electron beam and an optical light path simultaneously. Fluorescence microscope- highlight the regions of interest Electron microscope-obtain structural information at the nano- scale Carolien van Rijnsoever,Viola Oorschot Judith Klumperman,Nature Methods 5,973-980(2008) Pascal de Boer,Jacob P Hoogenboom Ben N G Giepmans,Nature Methsz,5(2015) School of Materials Science and Engineering材料科学与工程学院 School of Materials Science and Engineering Correlative Light and Electron Microscopy (CLEM) • In an integrated CLEM system, the sample is imaged using an electron beam and an optical light path simultaneously. Carolien van Rijnsoever, Viola Oorschot & Judith Klumperman, Nature Methods 5, 973 - 980 (2008) Pascal de Boer, Jacob P Hoogenboom & Ben N G Giepmans, Nature Methods 12, 503–513 (2015) Electron microscope- obtain structural information at the nano￾scale Fluorescence microscope￾highlight the regions of interest